Anti-IL-4 Antibody, clone 2D7.14.8

Interleukin-4 (UniProt: P05112; also known as IL-4, B-cell stimulatory factor 1, BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra) is encoded by the IL4 gene (Gene ID: 3565) in human. IL-4 is a cytokine that is secreted primarily by mast cells, Th2 cells, eosinophils, and basophils. IL-4 is synthesized with a signal peptide (1-24), which is subsequently cleaved off to produce the mature form. It participates in the activation of B cells and other cell types. It also serves as a co-stimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also reported to regulate the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. IL-4 inhibits macrophage production of proinflammatory cytokines, including TNF-a, IL-1, and IL-6 via transcriptional repression. It also enhances production of molecules that counteract these cytokines, such as IL-1R antagonist and the decoy receptor IL-1RII. The effects of IL-4 signaling are mediated through binding to the IL-4 receptor a chain (IL-4R ) that dimerizes with g-chain upon ligand binding to produce type I signaling complex located mainly of hematopoietic cells. It can also dimerize with IL-13a receptor a (IL-13Ra1) to produce type-2 complex that is expressed also on non-hematopoietic cells. IL-4 is also shown to stimulate autophagy in dendritic cells by interfering with mTORC1 signaling through the induction of RUN and FYVE domain containing 4 (RUFY4). (Ref.: Gadani, SP., et al. (2012). J. Immunol. 189(9); 4213-4219; Otsuka, TY., et al. (1986). Proc. Natl. Acad. Sci. USA. 83(16); 5894-5898).

Clone 2D7.14.8 is a mouse monoclonal antibody that detects human Interleukin-4.

Full-length recombinant human Interleukin-4 (without the signal peptide).

Quality Conrol Testing

Evaluated by Western Blotting with recombinant human IL-4

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected recombinant human IL-4.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-4 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-4, clone 2D7.14.8, Cat. No. MABF3181, is a mouse monoclonal antibody that detects human Inerleukin-4 and is is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Purified mouse monoclonal antibody IgG2a in PBS with 0.05% sodium azide.

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.