MABE253
抗-Ago2抗体，克隆11A9
clone 11A9, from rat
别名:
Protein argonaute-2, Argonaute2, hAgo2, Eukaryotic translation initiation factor 2C 2, eIF-2C 2, eIF2C 2, PAZ Piwi domain protein, PPD, Protein slicer
登录 查看组织和合同定价。
选择尺寸
关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
11A9, monoclonal
Application:
immunocytochemistry
immunoprecipitation (IP)
western blot
immunoprecipitation (IP)
western blot
Species reactivity:
human
Citations:
31
Technique(s):
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗-Ago2抗体，克隆11A9, clone 11A9, from rat
biological source
rat
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
11A9, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... AGO2(27161)
Analysis Note
对照
HeLa细胞裂解物
HeLa细胞裂解物
通过蛋白质印迹法在HeLa细胞裂解物中进行评估。
蛋白质印迹分析：该抗体以1:500稀释度在10 µg HeLa细胞裂解物中检测到Ago2。
蛋白质印迹分析：该抗体以1:500稀释度在10 µg HeLa细胞裂解物中检测到Ago2。
Application
使用在WB、IP、ICC中验证的抗Ago2抗体，克隆11A9（大鼠单克隆抗体）检测Ago2，也称为蛋白argonaute-2、Argonaute2、hAgo2、eIF-2C 2。
免疫沉淀分析：一个来自独立实验室的代表性批次在HEK239细胞裂解物中检测到Ago2（Rudel，S.，et al.（2008）RNA.14(6):1244-1253.)。
免疫细胞化学分析：一个来自独立实验室的代表性批次在HEK293、HeLa和RPE-1细胞中检测到Ago2（Weinmann，L.，et al.（2009）.Cell.136（3）:496-507.）。
免疫细胞化学分析：一个来自独立实验室的代表性批次在HEK293、HeLa和RPE-1细胞中检测到Ago2（Weinmann，L.，et al.（2009）.Cell.136（3）:496-507.）。
研究子类别
RNA代谢&结合蛋白
RNA代谢&结合蛋白
研究类别
表观遗传学&核功能
表观遗传学&核功能
Disclaimer
除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。
General description
Ago2（argonaute-2），也称为真核翻译起始因子2C（EIF2C2），是siRNA定向RNA干扰（RNAi）反应的重要组成部分。Ago2是解开siRNA双链体和将siRNA组装成RNA诱导的沉默复合物(RISC)所需的核酸内切酶。Ago2通过其Piwi域与DICER1交互。该Piwi结构域被认为通过类似于RNase H的机制提供RNA切割活性。Ago2活性对于胚胎发育以及RNA介导的基因沉默（RNAi）是必需的。
观测分子量〜85 kDa。已知存在约97 kDa和约94 kDa的两种亚型。
Immunogen
对应于人Ago2的线性肽。
Other Notes
浓度：关于批次特定浓度请参见检验报告。
Physical form
形式：纯化
纯化的大鼠单克隆IgG2aκ，溶于含0.1 M Tris-甘氨酸（pH 7.4、150 mM NaCl和0.05％叠氮化钠的缓冲液中。
纯化蛋白G
Preparation Note
自接收之日起，在2-8°C下可稳定保存1年。
未找到合适的产品？
试试我们的产品选型工具.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Tetsushi Iida et al.
Genes & genetic systems, 96(3), 107-118 (2021-06-11)
Many proteins form complexes that function in reaction pathways. Therefore, to understand protein function, it is necessary to reconstitute complexes and pathways in vitro. However, it is not straightforward to achieve full activity in reconstituted systems. To address this problem
Jihong Wen et al.
Oncology reports, 44(4), 1375-1384 (2020-09-19)
The long non‑coding RNA (lncRNA) MCM3AP antisense 1 (MCM3AP‑AS1) has previously been shown to be a key regulator of multiple types of cancer; however whether it is important in the context of ovarian cancer (OC) is uncertain. The present study
Meiling Chen et al.
International journal of molecular medicine, 47(1), 397-407 (2021-01-09)
Immature ovarian teratocarcinoma (IOT) is a rare and malignant type of ovarian teratoma, and the molecular mechanisms underlying the pathogenesis and malignant phenotype of IOT remain uncharacterized. The present study examined a long non‑coding RNA (lncRNA), long‑chain intergenic non‑coding RNA324
Davor Lessel et al.
Nature communications, 11(1), 5797-5797 (2020-11-18)
ARGONAUTE-2 and associated miRNAs form the RNA-induced silencing complex (RISC), which targets mRNAs for translational silencing and degradation as part of the RNA interference pathway. Despite the essential nature of this process for cellular function, there is little information on
Ravi K Patel et al.
Nucleic acids research, 48(17), 9724-9746 (2020-08-22)
The biological impact of microRNAs (miRNAs) is determined by their targets, and robustly identifying direct miRNA targets remains challenging. Existing methods suffer from high false-positive rates and are unable to effectively differentiate direct miRNA targets from downstream regulatory changes. Here
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持