Anti-FGFR4 Antibody, clone 4H2B10B2

FGFR4 (fibroblast growth factor receptor 4) is part of a family of fibroblast growth factor receptors that plays an important role in the biological functions of specific growth factors. The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation.

The previously assigned protein identifier G3JVM2 has been merged into P22455. Full details can be found on the UniProt database.

Purified recombinant fragment of human FGFR4 expressed in E. Coli.

Detect FGFR4 using this mouse monoclonal antibody, Anti-FGFR4 Antibody, clone 4H2B10B2 validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected FGFR4 in human liver tissue.

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Evaluated by Western Blotting in HEK293T cells transfected with the pCMV6-ENTRY control and pCMV6-ENTRY FGFR4 cDNA .

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected FGFR4 in HEK293T cells transfected with pCMV6-ENTRY FGFR4 cDNA.

~88 kDa observed

Unpurified

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
HEK293T cells transfected with the pCMV6-ENTRY control and pCMV6-ENTRY FGFR4 cDNA

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