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生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
1.B3.9B3, monoclonal
种属反应性
human, rat, pig
制造商/商品名称
Chemicon®
技术
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型
IgG1
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... CHAT(1103)
pig ... Chat(396896)
rat ... Chat(290567)
特异性
Reacts with choline acetyltransferase (ChAT)-positive cells from humans, rats and pigs (Ostermann-Fatif et al., 1992a,b).
免疫原
Highly purified ChAT obtained from porcine brain (Ostermann-Fatif et al., 1990).
应用
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
This Anti-Choline Acetyltransferase Antibody, clone 1.B3.9B3 is validated for use in ELISA, IH, IH(P), WB for the detection of Choline Acetyltransferase.
Western blot
ELISA
Immunohistochemistry: 10-20 μg/mL in paraffin embedded sections {See Ratcliffe et al. 1998}
Optimal working dilutions and protocols must be determined by end user.
ELISA
Immunohistochemistry: 10-20 μg/mL in paraffin embedded sections {See Ratcliffe et al. 1998}
Optimal working dilutions and protocols must be determined by end user.
目标描述
69 kDa
外形
Protein A purified
Format: Purified
Purified immunoglobulin. Lyophilized from 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide. Reconstitute to 100 μg/mL with sterile distilled water.
储存及稳定性
Maintain lyophilized antibody at 2–8°C in undiluted aliquots for up to 6 months. Maintain reconstituted antibody at -20°C in undiluted aliquots for up to 6 months.
分析说明
Control
Brain tissue
Brain tissue
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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