推荐产品
生物来源
mouse
质量水平
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
B10, monoclonal
种属反应性
rat, human
制造商/商品名称
Chemicon®
技术
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型
IgG2a
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... HSPG2(3339)
特异性
The peptide used as the immunogen for this product correspond to the amino-acid sequence 151-165 from human ER alpha receptor. By IP, western, gel retardation and ELISA assays clone B10 do not react with other nuclear receptors.
SPECIES REACTIVITIES:
Does not cross react with chicken ER. Other reactivities have not been evaluated. By sequence homology pig ER is 100% identical, rat is 80% conserved.
SPECIES REACTIVITIES:
Does not cross react with chicken ER. Other reactivities have not been evaluated. By sequence homology pig ER is 100% identical, rat is 80% conserved.
免疫原
Epitope: a.a. 151-165
应用
Immunohistochemistry (frozen sections, microwave oven treated paraffin sections) at 1:1,000-1:2,000
Immunocytochemistry (formaldehyde fixation) at 1:1,000-1:2,000
ELISA at 1:1,000-1:2,000
Western blot analysis at 1:1,000-1:2,000
Immunoprecipitation at 1:1-1:10
Gel super shifts at 1:1-1:10
Optimal working dilutions must be determined by the end user.
Immunocytochemistry (formaldehyde fixation) at 1:1,000-1:2,000
ELISA at 1:1,000-1:2,000
Western blot analysis at 1:1,000-1:2,000
Immunoprecipitation at 1:1-1:10
Gel super shifts at 1:1-1:10
Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-Estrogen Receptor Antibody, clone B10 (Mouse Monoclonal Antibody) validated in ELISA, WB, IHC(P) to detect Estrogen Receptor also known as ER.
外形
Ascites. Liquid, does not contain any preservative.
储存及稳定性
Maintain at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
P Bhat-Nakshatri et al.
British journal of cancer, 90(4), 853-859 (2004-02-19)
Oestrogen receptor alpha (ERalpha) is an oestrogen-activated transcription factor, which regulates proliferation and differentiation of mammary epithelial cells by activating or repressing gene expression. ERalpha is a critical prognostic indicator and a therapeutic target for breast cancer. Patients with tumours
Maie Dawoud Al-Bader et al.
Reproductive sciences (Thousand Oaks, Calif.), 22(4), 484-494 (2014-09-14)
Metastasis-associated protein 1 (MTA1) and its short form (MTA1s) regulate the function of estrogen receptors (ERs). Estrogens, via ERs, affect placental growth and fetal development, a process that may involve MTA1 signaling. Expression of MTA1, MTA1s, ERα, and ERβ genes
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门