Anti-BCRP Antibody, clone BXP-34

The 72 kDa breast cancer resistance protein (BCRP) is the second member of the subfamily G of the human ATP binding cassette (ABC) transporter superfamily and thus also designated as ABCG2. BCRP is a half-transporter consisting of only 1 nucleotide binding domain followed by 1 membrane-spanning domain. Current experimental evidence suggests that BCRP may function as a homodimer or homotetramer. Overexpression of BCRP is associated with high levels of resistance to a variety of anticancer agents. BCRP expression has been detected in a large number of hematological malignancies and solid tumors, indicating that this transporter may play an important role in clinical drug resistance of cancers. In addition to its role to confer resistance against chemotherapeutic agents, BCRP actively transports structurally diverse organic molecules, conjugated or unconjugated, such as estrone-3-sulfate, 17-β-estradiol 17-(β-D-glucuronide), and methotrexate. BCRP is highly expressed in the placental syncytiotrophoblasts, in the apical membrane of the epithelium in the small intestine, in the liver canalicular membrane, and at the luminal surface of the endothelial cells of human brain microvessels. This strategic and substantial tissue localization indicates that BCRP also plays an important role in absorption, distribution, and elimination of drugs that are BCRP substrates.

This antibody reacts with an internal epitope of the Breast Cancer Resistance Protein (BCRP), a 70 kDa transmembrane half-transporter which is involved in multidrug resistance. The antibody does not cross-react with human MDR1, MRP1, MRP2 or MRP5 gene products.

The Mitoxanthrone resistant, BCRP-overexpressing cell line MCF7-MR.

Detect BCRP using this Anti-BCRP Antibody, clone BXP-34 validated for use in IC, IH.

Immunocytochemistry: 1:20 - 1:50 on acetone fixed cytospin preparations

Immunohistochemistry: 1:20 on acetone fixed frozen tissue sections

Not suitable for paraffin-embedded tissues or for Western blotting

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research

Research Sub Category
Hematopoietic Stem Cells

70 kDa

Liquid containing 0.7% BSA and 0.1% sodium azide.

Maintain refrigerated at 2-8°C for up to 6 months. For long-term storage store at -20°C.

Control
MCF-7 cells

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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