产品名称
抗I型前胶原抗体,NT,克隆M-58, culture supernatant, clone M-58, Chemicon®
biological source
rat
antibody form
culture supernatant
clone
M-58, monoclonal
species reactivity
human
should not react with
rat, hamster, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
isotype
IgG2α
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Quality Level
Gene Information
human ... COL1A1(1277)
相关类别
Analysis Note
对照
皮肤组织
皮肤组织
Application
使用经验证可用于IH(P)的抗I型前胶原抗体(N-末端,克隆M-58)检测I型前胶原。
免疫组化:1:10,000-1:50,000(新鲜冰冻组织切片。)
适用于甲醛/福尔马林固定石蜡切片,建议初始稀释1:100-1:1000,用1%胰蛋白酶处理后,在室温下20分钟。 不染色组织中成熟的胶原纤维。在产生前胶原I的细胞内定位。不建议用于蛋白质印迹。最佳工作稀释度必须由最终用户确定。
适用于甲醛/福尔马林固定石蜡切片,建议初始稀释1:100-1:1000,用1%胰蛋白酶处理后,在室温下20分钟。 不染色组织中成熟的胶原纤维。在产生前胶原I的细胞内定位。不建议用于蛋白质印迹。最佳工作稀释度必须由最终用户确定。
Biochem/physiol Actions
识别人前胶原I。MAB1912标记氨基末端前肽。
Other Notes
浓度:请参考批次特异性浓缩物的分析证书。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 2
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