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Merck
CN

MAB1910

抗IV型胶原蛋白 α2链抗体(克隆23IIC3)

ascites fluid, clone 23IIC3, Chemicon®

别名:

Anti-BSVD2, Anti-ICH, Anti-POREN2

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
23IIC3, monoclonal
Application:
ELISA
immunohistochemistry
western blot
Species reactivity:
monkey, human, rat
Citations:
27
Technique(s):
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Uniprot accession no.:
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产品名称

抗IV型胶原蛋白 α2链抗体(克隆23IIC3), ascites fluid, clone 23IIC3, Chemicon®

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

23IIC3, monoclonal

species reactivity

monkey, human, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... COL4A2(1284)

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

170 kDa

Immunogen

人羊膜IV型胶原蛋白

Analysis Note

对照
阳性对照:肾脏、肌肉、肌腱、脾脏组织
阴性对照:神经元/神经胶质

Application

使用经验证可用于ELISA、IH(P)& WB的该抗IV型胶原蛋白α 2链抗体(克隆23IIC3)检测IIV型胶原蛋白α 2链。
免疫组化:丙酮固定、冷冻组织建议采用1:500。 在1%胰蛋白酶处理(20分钟,室温)后,在福尔马林固定的石蜡切片(1:100)上有微弱的反应性。 或者,4% PFA 固定、HEIR处理的组织在1:200稀释度下对猴有效(Qin, 2003)。

蛋白质印迹:1:500, 170 kDa.

1:200 的ELISA

最佳工作稀释度必须由最终用户进行确定。
研究子类别
ECM 蛋白
研究类别
细胞结构

Biochem/physiol Actions

识别人IV型胶原蛋白α-2链。

Other Notes

浓度:请参考批次特异性浓缩物的检验报告。

Physical form

未纯化
液体。

Preparation Note

自收到之日起在-20°C可稳定保存1年。避免反复冻融。为了最大程度地回收产品,在融化后和取下盖子之前,将原始小瓶进行离心。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Matteo Tagliapietra et al.
European journal of neurology, 30(12), 3834-3841 (2023-08-02)
Biallelic mutation/expansion of the gene RFC1 has been described in association with a spectrum of manifestations ranging from isolated sensory neuro(no)pathy to a complex presentation as cerebellar ataxia with neuropathy and vestibular areflexia syndrome (CANVAS). Our aim was to define
Peter Trosan et al.
PloS one, 13(3), e0194820-e0194820 (2018-03-28)
The human amniotic membrane (HAM) is widely used for its wound healing effect in clinical practice, as a feeder for the cell cultivation, or a source of cells to be used in cell therapy. The aim of this study was
Edward R Chu et al.
Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 30(2-3), 191-201 (2014-02-13)
We have developed a tissue-based model of the human trabecular meshwork (TM) using viable postmortem corneoscleral donor tissue. Two-photon microscopy is used to optically section and image deep in the tissue to analyze cells and extracellular matrix (ECM) within the
Epithelial organization and cyst lumen expansion require efficient Sec13-Sec31-driven secretion.
Townley, AK; Schmidt, K; Hodgson, L; Stephens, DJ
Journal of Cell Science null
Jasmin I Maier et al.
Cell reports, 34(12), 108883-108883 (2021-03-25)
The integrity of the kidney filtration barrier essentially relies on the balanced interplay of podocytes and the glomerular basement membrane (GBM). Here, we show by analysis of in vitro and in vivo models that a loss of the podocyte-specific FERM-domain protein EPB41L5

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