产品名称
抗肌动蛋白抗体,克隆C4, ascites fluid, clone C4, Chemicon®
生物来源
mouse
质量水平
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
C4, monoclonal
种属反应性(根据同源性预测)
all
制造商/商品名称
Chemicon®
技术
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型
IgG2bκ
NCBI登记号
UniProt登记号
靶向翻译后修饰
unmodified
基因信息
human ... ACTA1(58)
一般描述
免疫原
应用
细胞骨架信号转导
细胞结构
组织培养细胞--甲醛固定,甲醇或丙酮处理。
甘油化肌原纤维--用甲醛固定纤维,用冷甲醇处理。强烈染色人成纤维细胞中的I带和应激纤维。
低温恒温器切片(6µ m)-在异戊烷中快速冷冻,用明胶和甲醛处理的载玻片。
免疫印迹:
1:100-1:1,000(Otey,1987):在进行SDS-PAGE的肌肉匀浆上,与骨骼,心脏,砂囊和主动脉组织中存在的43 kD蛋白相对均匀地反应。似乎与这些制剂中发现的肌动蛋白的全部亚型反应,并显示与骨骼肌、心肌和动脉肌肉中发现的α-肌动蛋白强烈反应。
碘化(Lessard,1979)。
固相结合试验ELISA:
来自先前批次的1:800-1:1,000稀释液显示出与细胞质肌动蛋白强烈反应,并显示出与砂囊,骨骼,动脉和心脏肌动蛋白的显著结合。还显示出与盘基网柄菌和多头绒泡菌的显著结合。
ELISA:
与细胞质肌动蛋白强烈反应,并显示出与砂囊,骨骼,动脉和心脏肌动蛋白的显著结合。还显示出与盘基网柄菌和多头绒泡菌的显著结合。
最佳工作稀释度必须由最终用户进行确定。
生化/生理作用
外形
制备说明
分析说明
HeLa全细胞裂解物。
蛋白质印迹分析:
该批次以 1:500 的稀释度在 10ug A431裂解物中检测到肌动蛋白。
法律信息
免责声明
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
商品
Immunofluorescence uses antibody-conjugated fluorescent molecules for protein localization, modification confirmation, and protein complex visualization.
免疫荧光法(IF)通过在抗体标记上荧光分子,然后使用激光器激发荧光,包括蛋白质的定位、验证翻译后修饰或活化以及与其他蛋白质的邻近和复合等操作。
实验方案
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
相关内容
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Protein Blotting Survival Handbook: Tips and Tricks
Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.
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