Anti-Chymase Antibody, clone B7, biotin conjugated

Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.

Reacts with human mast cell chymase.

Chymase purified from human skin.

Immunohistochemistry at 1:2,000:1:4,000.

For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.

Not recommended for use on Western blots.

Optimal working dilutions must be determined by end user.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

This Anti-Chymase Antibody, clone B7, biotin conjugated is validated for use in DB, IH for the detection of Chymase.

Culture supernatant, purified by ammonium sulfate precipitation and DEAE-Sephacyl chromatography, labeled with biotin. In 0.01M PBS, 15 mg/mL BSA, 0.1% sodium azide, pH 7.1.

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Control
POSITIVE CONTROL: lung, heart, skin, placenta.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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