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主要文件

MAB10753

Sigma-Aldrich

抗半胱天冬酶3(活性形式)抗体,克隆3D9.3

clone 3D9.3, from mouse

别名:

Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

3D9.3, monoclonal

种属反应性

human

技术

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

同位素/亚型

IgMκ

NCBI登记号

UniProt登记号

一般描述

胱天蛋白酶-3(CPP32,Apopain)是研究最广泛的凋亡蛋白。胱天蛋白酶-3被合成为一种无活性的酶(32 kDa),该酶在经历凋亡的细胞中通过自身蛋白水解和/或被另一种上游蛋白酶切割而处理。胱天蛋白酶-3的加工形式由大(17 kDa)和小(12 kDa)亚基组成,它们结合形成活性酶。活性胱天蛋白酶-3蛋白水解切割并激活其他胱天蛋白酶,以及细胞中的相关靶标,例如PARP和DFF。

特异性

该抗体显示出对裂解的17 kDa活性形式的特异性,但不与35 kDa的非活性(原)形式的胱天蛋白酶3发生交叉反应。

免疫原

对应于人胱天蛋白酶3(活性形式)的KLH偶联线性肽。

应用

免疫细胞化学分析:代表性批次的1:500稀释液在Hela和Hela/星形孢菌素3小时细胞中检测到胱天蛋白酶3(活性形式)。
流式细胞术分析:代表性批次的1:400稀释液在Jurkat T细胞中检测到胱天蛋白酶3(活性形式)。
抗胱天蛋白酶3(活性形式),克隆3D9.3是一种靶向胱天蛋白酶3(活性形式)蛋白的抗体,经验证可用于WB、ICC&流。

质量

通过蛋白质印迹在HeLa 细胞裂解物中的进行了评估。

蛋白质印迹分析:0.1µ g/mL该抗体可在10µ g HeLa和星形孢菌素处理的HeLa细胞裂解物中检出胱天蛋白酶3(活性形式)。

目标描述

观测值〜17 kDa。胱天蛋白酶3在凋亡过程中裂解为17 kDa和12 kDa活性形式。

联系

替代品:04-1090;04-439

外形

形式:纯化

其他说明

浓度:请参考批次特异性浓缩物的分析证书。

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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