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生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
3D9.3, monoclonal
种属反应性
human
技术
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
同位素/亚型
IgMκ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... CASP3(836)
一般描述
胱天蛋白酶-3(CPP32,Apopain)是研究最广泛的凋亡蛋白。胱天蛋白酶-3被合成为一种无活性的酶(32 kDa),该酶在经历凋亡的细胞中通过自身蛋白水解和/或被另一种上游蛋白酶切割而处理。胱天蛋白酶-3的加工形式由大(17 kDa)和小(12 kDa)亚基组成,它们结合形成活性酶。活性胱天蛋白酶-3蛋白水解切割并激活其他胱天蛋白酶,以及细胞中的相关靶标,例如PARP和DFF。
特异性
该抗体显示出对裂解的17 kDa活性形式的特异性,但不与35 kDa的非活性(原)形式的胱天蛋白酶3发生交叉反应。
免疫原
对应于人胱天蛋白酶3(活性形式)的KLH偶联线性肽。
应用
免疫细胞化学分析:代表性批次的1:500稀释液在Hela和Hela/星形孢菌素3小时细胞中检测到胱天蛋白酶3(活性形式)。
流式细胞术分析:代表性批次的1:400稀释液在Jurkat T细胞中检测到胱天蛋白酶3(活性形式)。
流式细胞术分析:代表性批次的1:400稀释液在Jurkat T细胞中检测到胱天蛋白酶3(活性形式)。
抗胱天蛋白酶3(活性形式),克隆3D9.3是一种靶向胱天蛋白酶3(活性形式)蛋白的抗体,经验证可用于WB、ICC&流。
质量
通过蛋白质印迹在HeLa 细胞裂解物中的进行了评估。
蛋白质印迹分析:0.1µ g/mL该抗体可在10µ g HeLa和星形孢菌素处理的HeLa细胞裂解物中检出胱天蛋白酶3(活性形式)。
蛋白质印迹分析:0.1µ g/mL该抗体可在10µ g HeLa和星形孢菌素处理的HeLa细胞裂解物中检出胱天蛋白酶3(活性形式)。
目标描述
观测值〜17 kDa。胱天蛋白酶3在凋亡过程中裂解为17 kDa和12 kDa活性形式。
联系
替代品:04-1090;04-439
外形
形式:纯化
其他说明
浓度:请参考批次特异性浓缩物的分析证书。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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