PureProteome磁力架

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.

比较anti-FLAG® M2磁珠的小规模FLAG®标签蛋白纯化的不同洗脱方法。

Biomarkers offer important information about homeostasis, disease, response to drug treatments, and environmental stimuli. Sera are rich sources of biomarkers (biological indicator proteins, peptides, small molecules, etc.) and are easier to sample than other tissues. However, the complexity of serum and the presence of highly abundant proteins like albumin and immunoglobulin can mask less abundant species, hindering biomarker detection. PureProteome albumin magnetic beads remove more than 98% of albumin from human serum. Here, we demonstrate that PureProteome albumin magnetic beads may also be used to remove albumin from mouse, guinea pig and rat sera. Depleted samples are often dilute, and may need concentration for downstream analyses. Therefore, we present a protocol for the convenient concentration of these samples using Amicon Ultra 2 mL centrifugal filters.