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LIF2010

小鼠白血病抑制因子

Name: 小鼠白血病抑制因子
Brand: MM

10 µg, mouse recombinant LIF protein, expressed in E. coli, suitable for stem cell culture

别名:

LIF, Differentiation-stimulating Factor, D Factor, Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, mLIF

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About This Item

UNSPSC代码:

12352202

eCl@ss:

32160405

NACRES:

NA.75

生物来源

mouse

质量水平

300

检测方案

>95% (HPLC and SDS-PAGE)

形式

liquid

比活

≥1 x 10(E8) U/mg

制造商/商品名称

Chemicon^®

浓度

10 μg/mL

技术

cell culture | stem cell: suitable

杂质

<0.1 ng/mg Endotoxin (of LIF)

输入

sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)

NCBI登记号

NM_008501

UniProt登记号

P09056

运输

wet ice

应用

推荐的QC方案

M1生物测定

1.使用体外半固体琼脂培养物进行M1生物测定，该培养物在1 mL体积的DME（含20%FCS的0.3%琼脂）中含有约100个细胞。

2.加入100 μL样品或mLIF（等渗盐水中5%FCS中的10（E4）单位/mL），一式两份两倍系列稀释至35 mm培养皿。

3.将100μ L 5%FCS的等渗盐水加入到两个对照载玻片上。

4.在37°C、含10%CO2的完全湿润空气中培养7天。

5.对显示分化的菌落数进行评分（注：50单位定义为导致50%菌落分化的活性量）。

有关其他信息，请访问www.esgro-lif.com

LIF由CHEMICON International，Inc.生产，受美国专利号5,187,077、5,427,925、5,443,825、5,750,654 和 6,261,548，欧洲专利号 0285 448 和相关的外国专利的保护，并无法再销售。

单位定义

比活度：50 个单位定义为在 1 mL 琼脂培养物中诱导 50％ M1 克隆分化所需的小鼠 LIF 量。

分析说明

无菌和微质体测试均为阴性。

比活度：小鼠 LIF 的活性取决于诱导小鼠 M1 髓样白血病细胞分化的能力。在该测定法中，小鼠LIF的最低可检测浓度为 0.5 ng/mL

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Purification of embryonic stem cell-derived neurons by immunoisolation.

Kay Jüngling et al.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 17(14), 2100-2102 (2003-09-23)

The pluripotency and high proliferative capacity of embryonic stem (ES) cells (1-3) makes them an attractive source of different cell types for biomedical research and cell replacement therapies. A major prerequisite for these applications is the availability of a homogeneous

Cytochrome p450 cyp26a1 alters spinal motor neuron subtype identity in differentiating embryonic stem cells.

Megan J Ricard et al.

The Journal of biological chemistry, 288(40), 28801-28813 (2013-08-16)

The ability to differentiate embryonic stem cells (ESCs) into specific cell types is critical for improved regenerative medicine strategies, cancer chemotherapeutic approaches, and regimens to combat chronic diseases associated with aging. Subclasses of motor neurons (MNs) are generated at different

Murine Oncostatin M Acts via Leukemia Inhibitory Factor Receptor to Phosphorylate Signal Transducer and Activator of Transcription 3 (STAT3) but Not STAT1, an Effect That Protects Bone Mass.

Emma C Walker et al.

The Journal of biological chemistry, 291(41), 21703-21716 (2016-08-20)

Oncostatin M (OSM) and leukemia inhibitory factor (LIF) are IL-6 family members with a wide range of biological functions. Human OSM (hOSM) and murine LIF (mLIF) act in mouse cells via a LIF receptor (LIFR)-glycoprotein 130 (gp130) heterodimer. In contrast

Ciliary neurotrophic factor (CNTF) plus soluble CNTF receptor alpha increases cyclooxygenase-2 expression, PGE2 release and interferon-gamma-induced CD40 in murine microglia.

Hsiao-Wen Lin et al.

Journal of neuroinflammation, 6, 7-7 (2009-03-10)

Ciliary neurotrophic factor (CNTF) has been regarded as a potent trophic factor for motor neurons. However, recent studies have shown that CNTF exerts effects on glial cells as well as neurons. For instance, CNTF stimulates astrocytes to secrete FGF-2 and

An Improved System for Generation of Diploid Cloned Porcine Embryos Using Induced Pluripotent Stem Cells Synchronized to Metaphase.

Eunhye Kim et al.

PloS one, 11(7), e0160289-e0160289 (2016-07-30)

Pigs provide outstanding models of human genetic diseases due to their striking similarities with human anatomy, physiology and genetics. Although transgenic pigs have been produced using genetically modified somatic cells and nuclear transfer (SCNT), the cloning efficiency was extremely low.

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Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

实验方案

Mouse Embryonic Stem Cell Culture Procedures & Protocols

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

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