生物来源
human
质量水平
重组
expressed in Chem-1 cells
制造商/商品名称
ChemiScreen
Chemicon®
技术
radioligand binding assay (RLBA): suitable
NCBI登记号
UniProt登记号
运输
dry ice
基因信息
human ... CHRM2(1129)
1 of 4
此商品 | 107021 | PHR1219 | 90872 |
---|---|---|---|
grade ACS reagent, for extraction, for analytical purposes, reagent grade | grade ACS reagent, for analytical purposes, for extraction, reagent grade | grade certified reference material, pharmaceutical secondary standard | grade analytical standard |
assay ≥99.8% (GC) | assay ≥99.5% (GC) | assay - | assay ≥99.5% (GC) |
Quality Level 300 | Quality Level 200 | Quality Level 300 | Quality Level 100 |
application(s) environmental | application(s) environmental | application(s) pharmaceutical (small molecule) | application(s) cleaning products |
form liquid | form liquid | form liquid | form liquid |
vapor density 2 (vs air) | vapor density 2 (vs air) | vapor density 2 (vs air) | vapor density 2 (vs air) |
一般描述
生化/生理作用
质量
10 µg/well | |
---|---|
Signal:Background | 3.2 |
Specific Binding (cpm) | 606.8 |
Bmax for [3H]-NMS binding: 0.5 pmol/mg protein
Kd for [3H]-NMS binding: ~0.24 nM
产品规格
Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 4mM Na2HPO4, 1mM KH2PO4, pH7.4. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted. Binding and Wash buffer: 4mM Na2HPO4, 1mM KH2PO4, pH7.4, filtered and stored at 4°C Radioligand: [3H] NMS (Perkin Elmer#:NEX-636 ) One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 3-fold signal:background with 3H-labeled NMS at 0.5 nM
外形
Packaging method: Membranes protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80oC.
法律信息
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
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