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种属反应性
human
质量水平
制造商/商品名称
Milliplex®
技术
multiplexing: suitable
检测方法
fluorometric (Luminex® xMAP®)
储存温度
2-8°C
一般描述
Metabolic syndrome is a cluster of conditions that occur together; it includes increased blood pressure, elevated insulin levels, excess body fat around the waist and abnormal cholesterol levels.Key features of metabolic syndrome include insulin resistance, glucose intolerance, hypertension, dyslipidemia and central obsesity- all of which are risk factors for atherosclerosis, coronary heart disease, type 2 diabetes, kidney disease, and even premature death.Adults with metabolic syndrome show a low-grade inflammation, whose link with obesity may be dysregulated adipocyte production of pro- and anti-inflammatory factors. Consequently, research done in this area covers multi-faceted fields of cytokines, acute phase proteins, diabetes, and obesity related hormones, as well as other cardiovascular disease biomarkers.MILLIPLEX® Human Metabolic Hormone Panel V3 is a 14-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum, plasma, tissue/cell lysates, and culture supernatant samples: Amylin Active or Total, C-Peptide, Ghrelin Active, GIP, GLP-1 Active or Total, Glucagon, IL-6, Insulin, Leptin, MCP-1/CCL2, PP, PYY, Secretin and TNFα.Note: Amylin Active and Total cannot be run together in the same assay. GLP-1 Active an GLP-1 Total cannot be run together in the same assay.
特异性
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
应用
The Human Metabolic Hormone Panel V3, using the Luminex® xMAP technology, enables the simultaneous analysis of 14 protein biomarkers in human serum, plasma and tissue/lysate and culture supernatant samples.There are 16 biomarkers to choose from in this kit. However, this is a 14-plex kit since the following analytes cannot be run simultaneously: Amylin Active and Total cannot be run together in the same assay. GLP-1 Active and Total cannot be run together in the same assay.The following analytes require the addition of protease inhibitors to the serum or plasma samples (see sample collection and storage section in the protocol for details): GLP-1 (DPPIV inhibitor); Amylin (protease inhibitor cocktail); Glucagon (Aprotinin); Ghrelin (Active) (serine protease inhibitor). The protocol recommends serum or plasma samples be run neat. Tissue culture samples may be run neat or may require a dilution with an appropriate control medium. For optimal sensitivity, use an overnight (16 - 18 hour) incubation. Each well requires 25 uL of sample, standard or controlAnalytes available:Amylin (Total);C-Peptide;Ghrelin (Active);GIP;GLP-1 (Active);GLP-1 (Total);Glucagon;IL-6;Insulin;Leptin;MCP-1;PP;PYY;TNFα;Secretin;Amylin (Active)
特点和优势
Design your multiplex kit by choosing available analytes within this panel.
包装
96-well plate
储存及稳定性
Recommended storage for kit components is 2 - 8°C.
法律信息
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
警示用语:
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
WGK
WGK 3
法规信息
新产品
相关内容
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Multiplex immunoassays save time and sample volume in metabolic syndrome research, providing comprehensive insights into related conditions.
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