推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
BuGR2, monoclonal
形式
lyophilized
包含
≤0.1% sodium azide as preservative
种属反应性
guinea pig, rabbit, rat, mouse, sheep
请勿与下列物质发生反应
amphibian, primate, avian
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
同位素/亚型
IgG2a
运输
ambient
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
mouse ... Nr3C1(14815)
一般描述
Anti-Glucocorticoid Receptor (Ab-2), mouse monoclonal, clone BuGR2, recognizes ~94-97 kDa glucocorticoid receptor in mouse liver. It is validated for WB, ICC, IP & IHC (Frozen & paraffin sections).
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-AgX-653 myeloma cells (see application references). Recognizes the ~94-97 kDa glucocorticoid receptor.
Recognizes the ~94-97 kDa glucocorticoid receptor in mouse liver tissue.
免疫原
Rat
partially purified, rat glucocorticoid receptor
应用
Frozen Sections (5 g/ml)
Immunoblotting (5 g/ml)
Immunocytochemistry (5 g/ml)
Immunoprecipitation (see comments)
Paraffin Sections (5 g/ml)
警告
Toxicity: Standard Handling (A)
外形
Lyophilized from PBS, pH 7.2.
重悬
Resuspend the lyophilized antibody with 0.1 ml sterile PBS, pH 7.2. Be careful to reconstitute the entire contents of the vial; during shipment and handling, portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
分析说明
Positive Control
mouse liver extract
mouse liver extract
其他说明
Does not cross react with androgen, progesterone, estrogen or mineralocorticoid receptors. Enzymatic digestion and immunoblot analysis has shown that this antibody reacts with the undigested 97 kDa glucocorticoid receptor, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid and DNA-binding chymotrypsin fragment. Immunocytochemical localization of the glucocorticoid receptor is observed in the cytoplasm and nucleus, even in the presence of hormone. This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Hussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA94, 1521.
Pratt, W.B. and Toft, D.O., 1997. Endocrine Rev.18 306.
Schumacher, M., et al. 1996. Dev. Neurosci.18, 6.
Toran-Allerand, C.D. 1996. Dev. Neurosci.18, 36.
Weigel, N.L. 1996. Biochem J.319, 657.
Jones, K.J. 1994. Ann. N.Y. Acad. Sci.743, 141.
Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.
Pratt, W.B. and Toft, D.O., 1997. Endocrine Rev.18 306.
Schumacher, M., et al. 1996. Dev. Neurosci.18, 6.
Toran-Allerand, C.D. 1996. Dev. Neurosci.18, 36.
Weigel, N.L. 1996. Biochem J.319, 657.
Jones, K.J. 1994. Ann. N.Y. Acad. Sci.743, 141.
Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Mohammad M Khan et al.
Neuroendocrinology, 84(5), 317-329 (2006-12-05)
MNAR/PELP1 (see text) is a newly identified scaffold protein/coactivator initially thought to modulate nongenomic and genomic actions of the estrogen receptor; however, it has been recently shown to interact with multiple steroid receptors, including androgen and glucocorticoid receptors. In the
FoxA1 specifies unique androgen and glucocorticoid receptor binding events in prostate cancer cells.
Biswajyoti Sahu et al.
Cancer research, 73(5), 1570-1580 (2012-12-28)
The forkhead protein FoxA1 has functions other than a pioneer factor, in that its depletion brings about a significant redistribution in the androgen receptor (AR) and glucocorticoid receptor (GR) cistromes. In this study, we found a novel function for FoxA1
Nina M Patrick et al.
The Journal of steroid biochemistry and molecular biology, 167, 1-13 (2016-09-21)
Small molecule inhibitors of lysine deacetylases (KDACs) are approved for clinical use in treatment of several diseases. Nuclear receptors, such as the glucocorticoid receptor (GR) use lysine acetyltransferases (KATs or HATs) and KDACs to regulate transcription through acetylation and deacetylation
Vineela Kadiyala et al.
The Journal of biological chemistry, 288(40), 28900-28912 (2013-08-16)
Nuclear receptors use lysine acetyltransferases and lysine deacetylases (KDACs) in regulating transcription through histone acetylation. Lysine acetyltransferases interact with steroid receptors upon binding of an agonist and are recruited to target genes. KDACs have been shown to interact with steroid
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门