生物来源
human
质量水平
检测方案
>98% (SDS-PAGE)
制造商/商品名称
Chemicon®
技术
cell culture | mammalian: suitable
杂质
<0.1 ng/μg endotoxin (of PDGF-BB; 1EU/μg)
输入
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
UniProt登记号
运输
dry ice
基因信息
human ... PDGFB(5155)
一般描述
产品来源：大肠杆菌
分离自血小板的生长因子（PDGF）是一种有效的促细胞分裂剂，适用于多种细胞类型，包括成纤维细胞、平滑肌和结缔组织。由称为A链和B链形成的二聚体组成了PDGF，其可以AA或BB同二聚体或AB异二聚体形式存在。重组人PDGF-BB是由两条B链（共218个氨基酸）组成的24.3 kDa二硫键连接的同型二聚体。
应用
分离自血小板的生长因子（PDGF）是一种有效的促细胞分裂剂，适用于多种细胞类型，包括成纤维细胞、平滑肌 & 结缔组织。
对于大多数体外应用，PDGF-BB在0.2至10.0 ng/mL的浓度范围内发挥其生物学活性。响应的细胞包括（部分列表）：成纤维细胞、结缔组织。
联系
替代品：GF018
外形
从10 mM HOAc中冻干的无菌过滤溶液。
无菌的过滤溶液是从10mM HOAc中冻干得到。
储存及稳定性
冻干的PDGF-BB在室温下可稳定保存数周，但最好在-20°C下保存。快速离心后，在水中重溶至0.1-1.0 mg/mL的浓度。该溶液可以在缓冲水溶液中进行稀释，并可在2-8°C中保存一周或在-20°C保存供将来使用。重溶的PDGF-BB应以未稀释的等分形式保存于-20°C中，可稳定保存长达六个月。
分析说明
比活度：与标准品相比，人PDGF-BB具有完全的生物活性。通过其对BALB/c 3T3细胞的剂量依赖性有丝分裂活性所确定的ED50为6-12 ng/mL。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
WGK
WGK 1
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