生物来源
human
质量水平
检测方案
>97% (SDS-PAGE)
比活
≥2 x 107 U/mg
制造商/商品名称
Chemicon®
技术
cell culture | mammalian: suitable
输入
sample type hematopoietic stem cell(s)
NCBI登记号
UniProt登记号
运输
dry ice
基因信息
human ... TNF(7124)
相关类别
一般描述
产品来源：大肠杆菌
肿瘤坏死因子-α（TNF-α）是一种有效的淋巴样因子，可对多种肿瘤细胞和某些其他靶细胞发挥细胞毒性作用。人 TNF-α 的成熟形式具有 157 个氨基酸残基。 GF023 是重组多肽，其包含人 TNF-α 的成熟形式，其氨基末端具有多组氨酸标签。
应用
对于大多数体外应用，TNF-α 在 0.05 至 20.0 ng/mL 的浓度范围内发挥其生物学活性。响应细胞包括（部分列表）： 肿瘤细胞，某些其他细胞。
外形
从3mM Tris（pH 8.0）冻干。
储存及稳定性
冻干粉末尽管在室温下稳定，但最好在 -20°C 下干燥保存。用无菌蒸馏水稀释至 0.1-1 mg/mL。注意：使用前，让重悬后的小瓶在室温下放置 2 小时。可以将该溶液稀释到其他缓冲溶液中，并在 4°C 下保存 1 周或 -20°C 备用。重悬的 TNF-α 应该以未稀释的等分试样储存于 -20°C，最多保存六个月。
分析说明
比活度：与标准品相比，Chemicon′s 人 TNF-α具有完全的生物活性。在放线菌素-D 存在下，通过小鼠 L929 细胞的细胞溶解测定的 ED50≤ 0.05 ng/mL
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。
WGK
WGK 3
María Angélica Contreras et al.
The Biochemical journal, 477(17), 3299-3311 (2020-09-18)
TNFα is a pro-inflammatory cytokine that is a therapeutic target for inflammatory autoimmune disorders. Thus, TNFα antagonists are successfully used for the treatment of these disorders. Here, new association patterns of rhTNFα and its antagonists Adalimumab and Etanercept are disclosed.
María A Contreras et al.
Proteins, 89(11), 1557-1564 (2021-07-13)
The neutralization of tumor necrosis factor alpha (TNFα) with biopharmaceuticals is a successful therapy for inflammatory diseases. Currently, one of the main TNFα-antagonists is Etanercept, a dimeric TNF-R2 ectodomain. Considering that TNFα and its receptors are homotrimers, we proposed that
Sahar Rafat et al.
Biology, 11(11) (2022-11-12)
Cancer is the utmost common disease-causing death worldwide, characterized by uncontrollable cell division with the potential of metastasis. Overexpression of the Inhibitors of Apoptosis proteins (IAPs) and autophagy correlates with tumorigenesis, therapeutic resistance, and reoccurrence after anticancer therapies. This study
Hangeun Kim et al.
Methods in molecular biology (Clifton, N.J.), 1155, 125-132 (2014-05-03)
Hepatitis C virus (HCV) often causes chronic infection in humans, although the mechanisms for viral chronicity are not clearly understood. Tumor necrosis factor-α (TNF-α)-mediated apoptosis is a key element in a host organism's defense inhibiting viral spread and persistence. HCV
Rojo A Ratsimandresy et al.
Nature communications, 8, 15556-15556 (2017-06-06)
Inflammasomes are protein platforms linking recognition of microbe, pathogen-associated and damage-associated molecular patterns by cytosolic sensory proteins to caspase-1 activation. Caspase-1 promotes pyroptotic cell death and the maturation and secretion of interleukin (IL)-1β and IL-18, which trigger inflammatory responses to
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门