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安全信息

GF003AF

Sigma-Aldrich

Fibroblast Growth Factor basic, human

Animal free, >95% (SDS-PAGE), recombinant, expressed in E. coli, suitable for cell culture

别名:

FGF-2, bFGF, basic-FGF, Heparin-Binding Growth Factor 2, Prostatropin

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About This Item

UNSPSC代码:
12352202
eCl@ss:
32160405
NACRES:
NA.75

产品名称

成纤维细胞生长因子碱性，人重组，无动物, The Fibroblast Growth Factor-basic (or bFGF protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells.

生物来源

human

质量水平

100

方案

>95% (SDS-PAGE)

制造商/商品名称

Chemicon®

技术

cell culture | stem cell: suitable

杂质

<0.1 EU/μg Endotoxin (of bFGF)

输入

sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)

NCBI登记号

NM_002006.4

UniProt登记号

P09038

相关类别

一般描述

产品来源：在大肠埃希菌中表达
碱性成纤维细胞生长因子（bFGF）是一种肝素结合生长因子，可刺激多种细胞（包括间充质细胞，神经外胚层细胞和内皮细胞）的增殖。 bFGF还在体内发挥有效的血管生成活性。 人bFGF是一种含有155个氨基酸残基的17.2 kDa的蛋白。Xu等人证明bFGF可与BMP拮抗剂头蛋白协同作用，在无饲养层的条件下维持人胚胎干（hES）细胞的未分化增殖。 GF003AF是在没有基于动物的成分的情况下开发的，可用于hES细胞在无饲养层，无动物的培养系统中的培养。

应用

对于大多数体外应用，bFGF在0.1至10.0 ng/mL的浓度范围内发挥其生物学活性。 响应细胞包括（部分列表）： 内皮细胞，间充质细胞。根据培养的方法，人ES细胞所需的浓度在4到100 ng/ml的范围内

外形

从5mM Tris溶液（pH 7.6）和150mM NaCl溶液中冻干。

储存及稳定性

将冻干物料保持在-20°C，直至标签上注明的失效日期。

一般应用：
快速旋转后，在0.1 M磷酸盐缓冲液（pH值6.8）中复溶至0.1-1.0 mg/mL的浓度。 复溶的bFGF应以工作等分试样保存于-20°C中，可保存长达六个月。 多次冷冻/解冻循环会导致大量活性损失。

对于人ES细胞培养：
快速旋转后，在含有0.5%BSA，1 mM DTT和10%甘油的Dulbecco′s PBS过滤溶液中复溶至10 μg/mL。分装并在-20°C下储存长达6个月。 然后，该溶液可以解冻并稀释至4 ng/mL，用于人ES细胞与饲养层的培养，或稀释至8 ng/mL，以补充小鼠胚胎成纤维细胞调节培养基（用于无饲养层的人ES细胞培养）。

分析说明

比活度：与标准品相比，人FGF-b具有完全的生物活性。 ED50是通过表达FGF受体的NIH3T3细胞对胸苷摄取的剂量依赖性刺激来确定的。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

法规信息

监管及禁止进口产品

分析证书(COA)

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Hai Jin et al.
Journal of controlled release : official journal of the Controlled Release Society, 338, 773-783 (2021-09-17)
Pro-angiogenic growth factors have been studied as potential therapeutics for cardiovascular diseases like critical limb ischemia (CLI). However, the translation of these factors has remained a challenge, in part, due to problems associated with safe and effective delivery. Here, we
Mitra Aliabouzar et al.
Ultrasonics sonochemistry, 66, 105109-105109 (2020-04-06)
An ultrasound standing wave field (SWF) has been utilized in many biomedical applications. Here, we demonstrate how a SWF can enhance drug release using acoustic droplet vaporization (ADV) in an acoustically-responsive scaffold (ARS). ARSs are composite fibrin hydrogels containing payload-carrying
Leidan Huang et al.
Acta biomaterialia, 129, 73-83 (2021-05-09)
Vascularization is a critical step following implantation of an engineered tissue construct in order to maintain its viability. The ability to spatially pattern or direct vascularization could be therapeutically beneficial for anastomosis and vessel in-growth. However, acellular and cell-based strategies
Leiming Xia et al.
Cancer cell international, 21(1), 337-337 (2021-07-05)
Additional epidermal growth factor receptor (EGFR) mutations confer the drug resistance to generations of EGFR targeted tyrosine kinase inhibitor (EGFR-TKI), posing a major challenge to developing effective treatment of lung adenocarcinoma (LUAD). The strategy of combining EGFR-TKI with other synergistic
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Frontiers in immunology, 13, 1012594-1012594 (2022-10-18)
Obesity, prediabetes, and diabetes are growing in prevalence worldwide. These metabolic disorders are associated with neurodegenerative diseases, particularly Alzheimer's disease and Alzheimer's disease related dementias. Innate inflammatory signaling plays a critical role in this association, potentially via the early activation
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