跳转至内容
Merck
CN

FC010

Human Fibronectin

from human plasma, liquid, 1 mg/mL, purified protein, suitable for cell culture

别名:

纤连蛋白

登录 查看组织和合同定价。

选择尺寸

关于此项目

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

人血浆纤连蛋白纯化蛋白, from human plasma, liquid, 1 mg/mL (100 MG pack size is lyophilized), purified protein, suitable for cell culture

biological source

human

assay

~95% (SDS-PAGE)

form

(liquid: 1MG, 5MG, 10MG pack size
lyophilized: 100 MG pack size)

mol wt

220 kDa

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

surface coverage

2—10 μg/cm2

input

sample type mesenchymal stem cell(s)
sample type neural stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type pancreatic stem cell(s)

NCBI accession no.

NM_002026.2
NM_054034.2
NM_212474.1
NM_212475.1
NM_212476.1
NM_212478.1
NM_212482.1

UniProt accession no.

P02751

binding specificity

Peptide Source: Collagen

Peptide Source: Laminin

shipped in

wet ice

storage temp.

2-8°C

Quality Level

100

Gene Information

human ... FN1(2335)

相关类别

Analysis Note

220 kDa 的双带在还原条件下存在。hFN 是通过在明胶琼脂糖上的亲和层析，然后在肝素-琼脂糖上层析进行纯化的。已筛选出来自供体的血浆，并显示对 HIV、HTLV、乙型肝炎和丙型肝炎呈阴性。

Application

人纤连蛋白（HFN）当用于涂覆细胞培养表面（包括塑料制品、玻璃器皿和微载体珠子）时，适合用作体外细胞繁殖的附着因子。

Biochem/physiol Actions

纤连蛋白在各种细胞相互作用的介导中起关键作用，并参与各种过程，包括细胞粘附、细胞形态的建立和维持、细胞迁移、生长和分化。它与多种细胞外基质（ECM）和细胞表面蛋白（如胶原蛋白）、肝素、血纤维蛋白和特定细胞膜受体相互作用。纤连蛋白也可作为多种整联蛋白的的配体，包括总所周知的纤连蛋白受体 α5β1，该受体结合纤连蛋白III-10 重复区域中的精氨酰-甘氨酰-天冬氨酸序列。这种广泛的相互作用网络突显了纤连蛋白在调节细胞行为和介导组织功能中的重要性。

Disclaimer

仅供研究使用在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

纤连蛋白（FN）是细胞外基质（ECM）和体液中的高分子量粘着糖蛋白。它是由两个二硫键连接的两个亚基组成的二聚体。FN的每个单体由三个重复单元组成：12个I 型FN重复单元（每个单元含40个左右氨基酸）、两个II型重复单元（大约60个氨基酸）和15-17个III型重复单元（大约90个氨基酸）。这些重复单元有助于形成纤连蛋白分子结构和功能的多样性。

Other Notes

涂覆细胞培养皿的建议操作步骤

1.将总表面积（cm2）乘以目标HFN 浓度（μg/mL），确定覆盖培养皿所需的 HFN 量。建议用量为 2-10 μg/cm2.。

2.采用可涂覆整个培养皿的最少量的无菌平衡盐溶液（无血清和蛋白），润湿每个培养皿的表面。

3.如果需要，通入合适的CO2气氛。

4.将计算出的 HFN 量添加到每个培养皿中。

5.让 HFN 吸附到容器表面 5-20 分钟。

6.在进行标准细胞培养程序之前，去除残留的平衡盐溶液


在生产过程中，产品使用 0.1 微米过滤器进行过滤。建议在使用前/重悬后再次过滤产品。

Physical form

50 mM tris 缓冲盐水 (TBS) 溶液，pH 7.5，不含防腐剂。从 50 mM tris 缓冲盐水 (TBS) 冻干，pH 7.5，不含防腐剂（100 MG 包装规格）。37°C下使用无菌平衡盐溶液将整个 hFN 小瓶重悬为 1 mg/mL。

Preparation Note

自收到之日起，在2-8°C可最多保存6个月。 请勿冷冻。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

11 - Combustible Solids

wgk

WGK 3

法规信息

高风险级别生物产品-Merck
此项目有

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Integrin activation by dithiothreitol or Mn2+ induces a ligand-occupied conformation and exposure of a novel NH2-terminal regulatory site on the beta1 integrin chain.
Ni, H, et al.
The Journal of Biological Chemistry, 273, 7981-7987 (1998)
Intestinal epithelial wound healing assay in an epithelial-mesenchymal co-culture system.
Seltana, Amira, et al.
Wound Repair and Regeneration, 18, 114-122 null
Teemu Suutari et al.
Analytical chemistry, 92(21), 14509-14516 (2020-10-16)
Real-time label-free techniques are used to profile G protein-coupled receptor (GPCR) signaling pathways in living cells. However, interpreting the label-free signal responses is challenging, and previously reported methods do not reliably separate pathways from each other. In this study, a
Aleksander A Rebane et al.
FEBS letters, 594(7), 1132-1144 (2019-12-14)
Golgins are an abundant class of peripheral membrane proteins of the Golgi. These very long (50-400 nm) rod-like proteins initially capture cognate transport vesicles, thus enabling subsequent SNARE-mediated membrane fusion. Here, we explore the hypothesis that in addition to serving as
Sandrine B Lavenus et al.
The Journal of biological chemistry, 295(19), 6700-6709 (2020-04-03)
Tumor cells can spread to distant sites through their ability to switch between mesenchymal and amoeboid (bleb-based) migration. Because of this difference, inhibitors of metastasis must account for each migration mode. However, the role of vimentin in amoeboid migration has
查看所有相关文献

商品

Attachment Factors for Three-dimensional Cell Culture

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

什么是细胞外基质？

细胞外基质（ECM）是由细胞分泌并在组织中围绕在其周围。

查看所有结果

实验方案

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

查看所有结果

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持