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Merck
CN

EZHTNFA-150K

Human TNF-α ELISA Kit

detects TNF-α in 50 μL serum, plasma or cell culture supernatent

别名:

Human TNF-A ELISA, TNF-A ELISA, TNF-Alpha, Tumour Necrosis Factor Alpha ELISA

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UNSPSC Code:
41116158
NACRES:
NA.77
Input:
sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)
Species reactivity:
human
Storage temp.:
2-8°C
Shipped in:
wet ice

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产品名称

Human TNF-α Conferma® ELISA Kit,

species reactivity

human

packaging

kit of 1 × 96 wells

parameter

50 μL sample volume (4hr assay)

assay range

linearity: 89-113%
(Serum, 101%), linearity: 98-110%
(Plasma, 103%), recovery range: 81-111%
(Plasma, 97%), recovery range: 85-105%
(Serum,95%), inter-assay cv: ≤7%
intra-assay cv: ≤3%
sensitivity: 1.65 pg/mL
standard curve range: 1.65-400 pg/mL

technique(s)

ELISA: suitable

input

sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

description

kit sufficient for 96 wells

相关类别

General description

TNF-α is a core biomarker for the study of inflammation, considered to be a major contributing factor to numerous diseases and pathological processes. The Conferma® ELISA for TNF-α uses highly characterized critical reagents assessed by LC-MS, RPLC-UV-MS and Surface Plasmon Resonance (SPR) to deliver ensure similarity and that the mAb / Standard relationship is maintained . Each assay is further anchored against a recombinant gold standard and a sample library to ensure strong finished kit performance lot to lot. Detailed information can be found in the Protocol & Assay Verification Report.

Research Category: Inflammation

Research Sub-Category:
Alzheimer′s Disease
Aging
Inflammation
Neurodegenerative Diseases
Metabolic Disease
Sepsis Viral Diseases
Cardiovascular Disease

Application

For the detection of TNF-α by ELISA in Human Serum & Plasma.

Biochem/physiol Actions

This assay was tested for cross reactivity with other human cytokines like IL-1a, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17A, CCL3, CCL4, MCP-1, TNFα, GMCSF, VEGF. There is no cross-reactivity detected with any analyte.

Features and Benefits

Capture ELISA assay. Highly Verified. Suitable for 37 samples in Duplicate, with a 6 point Standard Curve, 3 Quality Controls and a Blank.

Legal Information

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

存储类别

8A - Combustible, corrosive hazardous materials

wgk

WGK 3

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商品

Conferma® ELISAs: Designed for Sample Detection & Lot Consistency

Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

实验方案

ELISA Protocols

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

ELISA实验方法

该文章提供了4种常用的ELISA实验方法：夹心法ELISA实验方法、磷酸化检测过程、EIA检测过程以及基于细胞的检测过程

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Protocol- EZHTNFA-150K

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