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CP18

Sigma-Aldrich

抗胶原II型(Ab-1)小鼠mAb(II-4C11)

liquid, clone II-4C11, Calbiochem®

别名:

Collagen Type II antibody

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.43

生物来源

mouse

质量水平

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

II-4C11, monoclonal

表单

liquid

包含

≤0.1% sodium azide as preservative

种属反应性

rat, human, bovine, rabbit

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

同位素/亚型

IgG1

运输

wet ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... COL2A1(1280)

一般描述

可识别约130 kDa的II型胶原蛋白。可能与其他胶原发生反应。当以高浓度使用时,也可以检测到对应于聚集体的更高条带。
经验证,该抗II型胶原(Ab-1)小鼠mAb(II-4C11)可用于冰冻切片检测II型胶原(Ab-1)。
通过用指定的免疫原免疫BALB/c小鼠并将脾细胞与P3-X-63-Ag8-LUI小鼠骨髓瘤融合而产生的纯化的小鼠单克隆抗体。可识别约130 kDa的II型胶原蛋白。

免疫原

纯化的II型胶原

应用

冷冻切片(1-10 µg/ml)

免疫印迹(1-5 µg/ml,请参阅应用参考)

石蜡切片(1-10 µg/ml,请参阅应用参考)

包装

请参考特定浓度批号的标签。

警告

毒性:标准处理(A)

外形

溶于100 mM磷酸钠缓冲液,0.1% BSA(pH 7.0)。

重悬

初次融化后,等分并冷冻(-20°C)。

其他说明

Kumagai, J. et al. 1994 J. Anat.185, 278.
Kivrikko, K,I.1993 Ann.Med.25, 113.
Sandell, L.J., et al. 1991 J. Cell.Biol.114, 1307.
Ryan, M.C., and Sandell, L.J.1990 J. Biol. Chem.265, 10334.
Mayne, R., and Burgeson, R.E.1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
可能与其他胶原发生反应。当以高浓度使用时,也可以检测到对应于聚集体的更高条带。该抗体也可以在高于1 µg/mL的浓度下与其他类型的胶原反应。在免疫印迹中,获得了约130 kDa的主要条带。在单个系统中,应对抗体进行滴定以获得最佳结果。

法律信息

由Daiichi Fine Chemical Co.,Ltd.生产,未在日本销售。
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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