生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
II-4C11, monoclonal
形式
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
rat, human, bovine, rabbit
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... COL2A1(1280)
一般描述
可识别约130 kDa的II型胶原蛋白。可能与其他胶原发生反应。当以高浓度使用时，也可以检测到对应于聚集体的更高条带。
经验证，该抗II型胶原（Ab-1）小鼠mAb（II-4C11）可用于冰冻切片检测II型胶原（Ab-1）。
通过用指定的免疫原免疫BALB/c小鼠并将脾细胞与P3-X-63-Ag8-LUI小鼠骨髓瘤融合而产生的纯化的小鼠单克隆抗体。可识别约130 kDa的II型胶原蛋白。
免疫原
人
纯化的II型胶原
应用
冷冻切片（1-10 µg/ml）
免疫印迹（1-5 µg/ml，请参阅应用参考）
石蜡切片（1-10 µg/ml，请参阅应用参考）
免疫印迹（1-5 µg/ml，请参阅应用参考）
石蜡切片（1-10 µg/ml，请参阅应用参考）
包装
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
外形
溶于100 mM磷酸钠缓冲液，0.1% BSA（pH 7.0）。
重悬
初次融化后，等分并冷冻（-20°C）。
其他说明
Kumagai, J. et al. 1994 J. Anat.185, 278.
Kivrikko, K,I.1993 Ann.Med.25, 113.
Sandell, L.J., et al. 1991 J. Cell.Biol.114, 1307.
Ryan, M.C., and Sandell, L.J.1990 J. Biol. Chem.265, 10334.
Mayne, R., and Burgeson, R.E.1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
Kivrikko, K,I.1993 Ann.Med.25, 113.
Sandell, L.J., et al. 1991 J. Cell.Biol.114, 1307.
Ryan, M.C., and Sandell, L.J.1990 J. Biol. Chem.265, 10334.
Mayne, R., and Burgeson, R.E.1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
可能与其他胶原发生反应。当以高浓度使用时，也可以检测到对应于聚集体的更高条带。该抗体也可以在高于1 µg/mL的浓度下与其他类型的胶原反应。在免疫印迹中，获得了约130 kDa的主要条带。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律信息
由Daiichi Fine Chemical Co.，Ltd.生产，未在日本销售。
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our 产品选型工具.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Silencing of Wnt5a prevents interleukin-1β-induced collagen type II degradation in rat chondrocytes.
Shiping Shi et al.
Experimental and therapeutic medicine, 12(5), 3161-3166 (2016-11-25)
Osteoarthritis (OA) is a joint disease, and few treatments to date have been able to delay OA progression. The degradation of collagen type II (COL2) in the cartilage matrix is an important initiating factor for OA progression; the upregulation of
Han-Jun Kim et al.
Oxidative medicine and cellular longevity, 2020, 5915481-5915481 (2020-10-09)
Intervertebral disc degeneration (IVDD) is a common cause of lower back pain. Programmed cell death (PCD) including apoptosis and autophagy is known to play key mechanistic roles in the development of IVDD. We hypothesized that the nucleus pulposus cells that
Zheng-Zheng Zhang et al.
Science translational medicine, 11(487) (2019-04-12)
Reconstruction of the anisotropic structure and proper function of the knee meniscus remains an important challenge to overcome, because the complexity of the zonal tissue organization in the meniscus has important roles in load bearing and shock absorption. Current tissue
Chang Qing et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 44(4), 303-310 (2011-04-14)
Chondrocytes and bone marrow mesenchymal stem cells (BMSCs) are frequently used as seed cells in cartilage tissue engineering. In the present study, we determined if the co-culture of rabbit articular chondrocytes and BMSCs in vitro promotes the expression of cartilaginous
Johannes Kupka et al.
International journal of molecular sciences, 21(6) (2020-03-22)
Healthy and degenerating intervertebral discs (IVDs) are innervated by sympathetic nerves, however, adrenoceptor (AR) expression and functionality have never been investigated systematically. Therefore, AR gene expression was analyzed in both tissue and isolated cells from degenerated human IVDs. Furthermore, human
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门