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Merck
CN
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文件

CC130

Sigma-Aldrich

Human Vitronectin

recombinant, expressed in E. coli, liquid, 0.5 mg/mL, used as an effective xeno-free alternative to matrigel, suitable for human ES and iPS cell culture, PLURISTEM®

别名:

S-protein, Serum-spreading factor, V75

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About This Item

UNSPSC代码:
12352202
eCl@ss:
32160405
NACRES:
NA.75

product name

PluriSTEM-XF® 重组玻连蛋白, Human pluripotent stem cell culture.

生物来源

Escherichia coli
human

质量水平

100

检测方案

≥92% (SDS-PAGE)

形式

liquid

制造商/商品名称

PluriSTEM®

浓度

0.5 mg/mL

技术

cell attachment: suitable
cell culture | mammalian: suitable
cell culture | stem cell: suitable

输入

sample type: human embryonic stem cell(s)

UniProt登记号

P04004

运输

dry ice

相关类别

一般描述

PluriSTEM 重组玻连蛋白是matrigel的有效无异种替代品，已被验证可以使用PluriSTEM人ES/iPS培养基（Millipore目录编号SCM130，SCM132），E8和NutriStem人ES细胞生长培养基成功培养人ES和iPS细胞。

PluriSTEM重组玻连蛋白是人玻连蛋白（基因20-398aa片段）的截短形式，其通过密码子优化构建，在大肠埃希菌中表达并纯化为包含体。使用独特的“温度变化包涵体重折叠”技术将最终产物重折叠并进行色谱纯化。最终产品被认为是无异种的。

应用

支持人es/ips细胞的无饲养层和无血清生长，同时维持无异种培养条件。
研究类别
干细胞研究

细胞结构

ECM & 粘连

质量

在每孔1 mL人ESC培养基（6孔板）中使用5-10 μg重组玻连蛋白培养的人ES细胞（H1）上测试每个批次。

外形

在含有专有处方NaCl、KCl、EDTA、精氨酸、DTT和甘油的20 mM pH值8.0 Tris-HCl缓冲液中对产品进行无菌过滤。

储存及稳定性

储存于-20°C，直到小瓶上的有效期为止。 解冻后，产品在2-8°C下可稳定储存两周。 解冻后的玻连蛋白可分装于-20°C保存。 应避免其他冻融循环。

法律信息

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Mutsumi Kobayashi et al.
Stem cell reports, 17(3), 507-521 (2022-02-13)
In vitro expansion of human primordial germ cell-like cells (hPGCLCs), a pluripotent stem cell-derived PGC model, has proved challenging due to rapid loss of primordial germ cell (PGC)-like identity and limited cell survival/proliferation. Here, we describe long-term culture hPGCLCs (LTC-hPGCLCs), which

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