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Merck
CN

CC077

人胶原V型

from human placenta, liquid, 1 mg/mL, suitable for cell culture, used for gel formation

别名:

V型胶原蛋白

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关于此项目

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

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产品名称

人胶原V型, 1 mg/mL, solution, BioReagent, suitable for cell culture

biological source

human

assay

95% (SDS-PAGE)

form

solution

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

impurities

<0.5% non-collagen proteins
<1% collagen type III
<2% collagen type I
<2% collagen type IV

input

sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type pancreatic stem cell(s)
sample type epithelial cells
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type: human embryonic stem cell(s)

NCBI accession no.

NM_000093.3

UniProt accession no.

P20908

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Gene Information

human ... COL5A1(1289), COL5A2(1290)

相关类别

Application

人类V型胶原蛋白已用于荧光偏振(FP)分析和表面等离子共振(SPR)技术研究高内皮细胞微静脉蛋白(high endothelial venule protein, hevin)和人V型胶原的相互作用。还用于孵育96孔板，用于测定基质和成纤维细胞裂解物中的蛋白水平。

Biochem/physiol Actions

V型胶原可调节角膜和皮肤真皮中的胶原纤维形成。与基质胶原和结构蛋白相互作用，共同维持组织的结构完整性。V型胶原水平偏低会导致角膜透明度的丧失和Ehlers-Danlos综合征（皮肤弹性过度综合征）。

Disclaimer

仅供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

General description

V型胶原是纤维型胶原蛋白，存在于肺、骨、胎膜中，与I型胶原共分布。位于细胞外基质，含量偏低。

Other Notes

分子组成：α1(V)]2 α2(V),天然三螺旋。通过SDS-PAGE，ORD测量以及与抗胶原类型特异性单克隆抗体的反应来监测天然螺旋结构的纯度和保留。产品来源：人胎盘，HBsAg和HIV抗体阴性

Physical form

液体，在0.1 M醋酸（pH 3.0）中。 没有添加防腐剂。

Preparation Note

将未稀释的分装样品保存于-20°C，最长12个月。请勿解冻和重新冷冻。
通过人胎膜胃蛋白酶提取物的连续盐沉淀和DEAE-纤维素色谱纯化。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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The effects of tissue pretreatment and pepsin levels on the isolation of collagens from human placenta.
Klasson, S C, et al.
Collagen and Related Research, 6, 397-408 (1986)
Type V collagen in health, disease, and fibrosis
The Anatomical Record, 299(5), 613-629 (2016)
K Gelse et al.
Advanced drug delivery reviews, 55(12), 1531-1546 (2003-11-19)
The extracellular matrix represents a complex alloy of variable members of diverse protein families defining structural integrity and various physiological functions. The most abundant family is the collagens with more than 20 different collagen types identified so far. Collagens are
Neena Philips et al.
Connective tissue research, 53(5), 373-378 (2012-02-14)
Skin aging is associated with the loss of the structural collagens and the elastin fiber components that form the extracellular matrix (ECM). It is associated with reduced transforming growth factor-β (TGF-β), angiogenesis and increased oxidative stress. Copper has been incorporated
Shanghua Fan et al.
Structure (London, England : 1993), 29(7), 664-678 (2021-02-04)
Hevin is secreted by astrocytes and its synaptogenic effects are antagonized by the related protein, SPARC. Hevin stabilizes neurexin-neuroligin transsynaptic bridges in vivo. A third protein, membrane-tethered MDGA, blocks these bridges. Here, we reveal the molecular underpinnings of a regulatory network
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实验方案

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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