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product name
人胶原V型,
生物来源
human
质量水平
检测方案
95% (SDS-PAGE)
形式
liquid
制造商/商品名称
Chemicon®
浓度
1 mg/mL
技术
cell culture | mammalian: suitable
杂质
<0.5% non-collagen proteins
<1% collagen type III
<2% collagen type I
<2% collagen type IV
输入
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type pancreatic stem cell(s)
sample type epithelial cells
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type: human embryonic stem cell(s)
NCBI登记号
UniProt登记号
运输
dry ice
储存温度
−20°C
基因信息
human ... COL5A1(1289) , COL5A2(1290)
一般描述
V型胶原是纤维型胶原蛋白，存在于肺、骨、胎膜中，与I型胶原共分布。位于细胞外基质，含量偏低。
应用
人类V型胶原蛋白已用于荧光偏振(FP)分析和表面等离子共振(SPR)技术研究高内皮细胞微静脉蛋白(high endothelial venule protein, hevin)和人V型胶原的相互作用。还用于孵育96孔板，用于测定基质和成纤维细胞裂解物中的蛋白水平。
生化/生理作用
V型胶原可调节角膜和皮肤真皮中的胶原纤维形成。与基质胶原和结构蛋白相互作用，共同维持组织的结构完整性。V型胶原水平偏低会导致角膜透明度的丧失和Ehlers-Danlos综合征（皮肤弹性过度综合征）。
外形
液体，在0.1 M醋酸（pH 3.0）中。 没有添加防腐剂。
制备说明
通过人胎膜胃蛋白酶提取物的连续盐沉淀和DEAE-纤维素色谱纯化。
储存及稳定性
将未稀释的分装样品保存于-20°C，最长12个月。请勿解冻和重新冷冻。
其他说明
分子组成：α1(V)]2 α2(V),天然三螺旋。通过SDS-PAGE，ORD测量以及与抗胶原类型特异性单克隆抗体的反应来监测天然螺旋结构的纯度和保留。产品来源：人胎盘，HBsAg和HIV抗体阴性
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Type V collagen in health, disease, and fibrosis
The Anatomical Record, 299(5), 613-629 (2016)
The effects of tissue pretreatment and pepsin levels on the isolation of collagens from human placenta.
Klasson, S C, et al.
Collagen and Related Research, 6, 397-408 (1986)
K Gelse et al.
Advanced drug delivery reviews, 55(12), 1531-1546 (2003-11-19)
The extracellular matrix represents a complex alloy of variable members of diverse protein families defining structural integrity and various physiological functions. The most abundant family is the collagens with more than 20 different collagen types identified so far. Collagens are
Neena Philips et al.
Connective tissue research, 53(5), 373-378 (2012-02-14)
Skin aging is associated with the loss of the structural collagens and the elastin fiber components that form the extracellular matrix (ECM). It is associated with reduced transforming growth factor-β (TGF-β), angiogenesis and increased oxidative stress. Copper has been incorporated
Shanghua Fan et al.
Structure (London, England : 1993), 29(7), 664-678 (2021-02-04)
Hevin is secreted by astrocytes and its synaptogenic effects are antagonized by the related protein, SPARC. Hevin stabilizes neurexin-neuroligin transsynaptic bridges in vivo. A third protein, membrane-tethered MDGA, blocks these bridges. Here, we reveal the molecular underpinnings of a regulatory network
实验方案
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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