产品名称
人胶原蛋白 Ⅰ 型, 1 mg/mL, solution, BioReagent, suitable for cell culture
biological source
human
assay
>90% (collagen type I, SDS-PAGE)
form
liquid
solution
manufacturer/tradename
Chemicon®
concentration
1 mg/mL
technique(s)
cell culture | mammalian: suitable
impurities
<1% collagen type II,IV-VI & non-collagen proteins.
<10% collagen type III
input
sample type pancreatic stem cell(s)
sample type mesenchymal stem cell(s)
sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type: human embryonic stem cell(s)
sample type hematopoietic stem cell(s)
solubility
water: soluble at 20 °C
NCBI accession no.
UniProt accession no.
binding specificity
Peptide Source: Elastin
Peptide Source: Fibronectin
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
human ... COL1A1(1277)
Biochem/physiol Actions
Disclaimer
Analysis Note
Application
- 活化T细胞2B4核因子(NFAT))–GFP报告基因细胞检测中的对照品,在该检测中其与报告基因细胞的相互作用可以评估活化T细胞核因子(NFAT)启动子驱动的GFP表达
- 在动态结合测定中包被玻璃载玻片,以构建特异性结合的底物并研究流动通道中的血小板和结合物
- 作为肺组织样品组织学分析中的蛋白质标准品,为细胞外基质(ECM)成分的组成和标准提供参考。
General description
Physical form
Legal Information
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
细胞外基质蛋白质,如层粘连蛋白、胶原蛋白和纤连蛋白,可用作细胞培养中的细胞附着基底。
实验方案
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
相关内容
As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.
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