Capsaicin Receptor Control Peptide for AB5889

FUNCTION: SwissProt: Q8NER1 # Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL.
SIZE: 839 amino acids; 94938 Da
SUBUNIT: Self-associates. Probably homotetramer. May also form a heteromeric channel with TRPV3. Interacts with calmodulin, PRKCM and CSK. Interacts with PRKCG and NTRK1, probably by forming a trimeric complex (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).DOMAIN:SwissProt: Q8NER1 The association domain (AD) is necessary for self- association (By similarity).
PTM: Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity (By similarity).
SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. & Contains 3 ANK repeats.
MISCELLANEOUS: Responses evoked by low pH and heat, and capsaicin can be antagonized by capsazepine.

Antibody blocking. Antiserum (Catalog No. AB5889) should be preincubated with peptide prior to incubation with tissue/sample. A 10^-5 molar (M) peptide concentration is suggested to block antibody/antigen binding.

Optimal working dilution must be determined by the end user.

Lyophilized in phosphate buffer, pH 7.4.

Store at -20°C or below in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

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