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ABT168

Sigma-Aldrich

Anti-Girdin Antibody

from rabbit, purified by affinity chromatography

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UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

rabbit

质量水平

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

纯化方式

affinity chromatography

种属反应性

human, monkey

种属反应性(根据同源性预测)

rat (immunogen homology)

技术

immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

NCBI登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... CCDC88A(55704)

一般描述

Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells.

特异性

Other homologies: Mouse (97% sequence homology).
This antibody recognizes the coiled-coil domain of Girdin.

免疫原

Epitope: Coiled coil domain.
GST-tagged recombinant protein corresponding to the coiled coil domain rat Girdin.

应用

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Girdin in human colorectal cancer tissues.

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Girdin in HeLa, Cos-7, and Att20 cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in HeLa cell lysate.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Girdin antibody is validated for use in WB, IHC, IF & IP for the detection of the Girdin protein.

质量

Evaluated by Western Blotting in Cos-7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Girdin in 10 µg of Cos-7 cell lysate.

目标描述

~220 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

外形

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Cos-7 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Pradipta Ghosh et al.
Current protocols in chemical biology, 8(4), 265-298 (2016-12-08)
Canonical signal transduction via heterotrimeric G proteins is spatiotemporally restricted, i.e., triggered exclusively at the plasma membrane, only by agonist activation of G protein-coupled receptors via a finite process that is terminated within a few hundred milliseconds. Recently, a rapidly

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