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Merck
CN

ABS14

Anti-PKA RII Beta Antibody

from rabbit, purified by affinity chromatography

别名:

protein kinase, cAMP-dependent, regulatory, type II, beta, cAMP-dependent protein kinase type II-beta regulatory chain, cAMP-dependent protein kinase type II-beta regulatory subunit

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
western blot
Species reactivity:
mouse, human, horse
Citations:
2
Technique(s):
western blot: suitable
Uniprot accession no.:
P31323

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产品名称

Anti-PKA RII Beta Antibody, from rabbit, purified by affinity chromatography

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human, horse

species reactivity (predicted by homology)

bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), canine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), equine (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

NP_002727

UniProt accession no.

P31323

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... PRKAR2B(5577)

Application

Research Sub Category
Kinases & Phosphatases
Anti-PKA RII Beta Antibody is an antibody against PKA RII Beta for use in WB.
Research Category
Signaling
Western Blot Analysis: 0.5 µg/mL from a previous lot detected PKA RII Beta on 10 µg of mouse brain tissue lysate.

Analysis Note

Control
Human brain tissue lysate
Evaluated by Western Blot in human brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PKA RII Beta on 10 µg of human brain tissue lysate.

Biochem/physiol Actions

This antibody recognizes PKA RII Beta at the cAMP2 domain.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PKA RII Beta is a type two regulatory chain which mediates membrane association by binding to anchoring proteins, including the MAP2 kinase. When the enzyme is inactive there are two regulatory chains and two catalytic chains. PKA RII Beta is activated by cAMP and produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules.
~ 46 kDa observed

Immunogen

Epitope: cAMP2 domain
KLH-conjugated linear peptide corresponding to human PKA RII Beta at the cAMP2 domain.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Jacky Chung et al.
eLife, 6 (2017-05-30)
Heme is required for survival of all cells, and in most eukaryotes, is produced through a series of eight enzymatic reactions. Although heme production is critical for many cellular processes, how it is coupled to cellular differentiation is unknown. Here

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