AB758
Anti-Collagen Type I (COL1A1) Antibody
CHEMICON®, goat polyclonal
别名:
Anti-CAFYD, Anti-EDSARTH1, Anti-EDSC, Anti-OI1, Anti-OI2, Anti-OI3, Anti-OI4
产品名称
Anti-Collagen Type I Antibody, Chemicon®, from goat
biological source
goat
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, bovine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
suitability
not suitable for immunohistochemistry (Paraffin)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... COL1A1(1277)
Application
Anti-Collagen Type I Antibody is an antibody against Collagen Type I for use in DB, ELISA, IC, IH & WB.
Dot and slot blotting: 1:100-1:500
ELISA: 1:50-1:100
Indirect immunohistochemistry (frozen sections only): 1:10-1:20
Indirect immunocytochemistry: 1:10-1:20
Antibody reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts {LMF, personal communication}.
Not recommended for paraffin tissue sections.
Optimal working dilutions must be determined by the end user.
ELISA: 1:50-1:100
Indirect immunohistochemistry (frozen sections only): 1:10-1:20
Indirect immunocytochemistry: 1:10-1:20
Antibody reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts {LMF, personal communication}.
Not recommended for paraffin tissue sections.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).
Immunogen
Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen types II, III, IV, V and VI immobilized on Sepharose™ 4B.. Liquid in 0.5 mL of 100 mM borate buffered saline, pH 8.0, no preservatives.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva
未找到合适的产品？
试试我们的产品选型工具.
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
存储类别
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Felix Dyrna et al.
The American journal of sports medicine, 46(14), 3511-3520 (2018-11-13)
Bone marrow aspirate is a primary source for cell-based therapies with increasing value in the world of orthopaedic surgery, especially in revision cases of tendon and ligament repairs. However, cells within peritendinous structures, such as the paratenon and surrounding bursa
Andrew P Breidenbach et al.
Tissue engineering. Part A, 21(3-4), 438-450 (2014-10-01)
The prevalence of tendon and ligament injuries and inadequacies of current treatments is driving the need for alternative strategies such as tissue engineering. Fibrin and collagen biopolymers have been popular materials for creating tissue-engineered constructs (TECs), as they exhibit advantages
Nannan Guo et al.
Frontiers in immunology, 11, 1466-1466 (2020-08-09)
Imaging mass cytometry (IMC) is able to quantify the expression of dozens of markers at sub-cellular resolution on a single tissue section by combining a novel laser ablation system with mass cytometry. As such, it allows us to gain spatial
Andrea L Lalley et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 33(11), 1693-1703 (2015-05-20)
Musculoskeletal injuries greatly affect the U.S. population and current clinical approaches fail to restore long-term native tissue structure and function. Tissue engineering is a strategy advocated to improve tendon healing; however, the field still needs to establish biological benchmarks for
Eva Crosas-Molist et al.
Arteriosclerosis, thrombosis, and vascular biology, 35(4), 960-972 (2015-01-17)
Marfan's syndrome is characterized by the formation of ascending aortic aneurysms resulting from altered assembly of extracellular matrix microfibrils and chronic tissue growth factor (TGF)-β signaling. TGF-β is a potent regulator of the vascular smooth muscle cell (VSMC) phenotype. We
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持