产品名称
抗胶质纤维酸性蛋白抗体, Chemicon®, from chicken
biological source
chicken
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse, rat, bovine, pig
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GFAP(2670)
Analysis Note
蛋白质印迹分析:该批次的1:1000稀释液在10 μg小鼠脑裂解物中检测到胶质纤维酸性蛋白。
星形胶质细胞,星形细胞瘤,神经干细胞。
Application
先前批次的代表性图像。柠檬酸盐缓冲液表位修复的最佳染色图片: 人脑。
免疫细胞化学:
在组织培养中的细胞上为1:200-1:1,000,细胞在3.7%福尔马林中固定一分钟,在-20°C甲醇中固定一分钟。
蛋白质印迹:
使用ECL为1:100-1:500识别55 kDa的条带。也可能与50 kDa的条带反应。
免疫组化:
在冰冻组织切片上为1:100-1:500。建议的固定剂为4%多聚甲醛。
最佳工作稀释度必须由最终用户进行确定。
染色模式:
该抗体染色组织培养中星形胶质细胞的清晰定义的细胞质丝。染色脑组织切片中星形胶质细胞的过程。
Biochem/physiol Actions
General description
Immunogen
Physical form
Preparation Note
处理建议:
第一次融化后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。 分装到微量离心管中,并储存于 -20°C。 避免反复冻融循环,以免损坏 IgG 和影响产品性能。
Legal Information
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存储类别
10 - Combustible liquids
wgk
WGK 2
商品
Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.
Learn differences in monoclonal vs polyclonal antibodies including how antibodies are generated, clone numbers, and antibody formats.
Immunofluorescence uses antibody-conjugated fluorescent molecules for protein localization, modification confirmation, and protein complex visualization.
Explore the basics of working with antibodies including technical information on structure, classes, and normal immunoglobulin ranges.
实验方案
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
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