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生物来源
rabbit
质量水平
抗体形式
affinity purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
rat
制造商/商品名称
Chemicon®
技术
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
rat ... Cacna1G(29717)
特异性
Recognizes Cav3.1. The epitope specific for Cav3.1 is not present in any other known proteins.
SPECIES REACTIVITIES:
The immunogen sequence is conserved in human (20/22). Reactivity with other species has not yet been tested.
SPECIES REACTIVITIES:
The immunogen sequence is conserved in human (20/22). Reactivity with other species has not yet been tested.
免疫原
Highly purified peptide corresponding to residues 1-22 of rat Cav3.1 (Accession O54898).
应用
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Neuroinflammation & Pain
Ion Channels & Transporters
Neuroinflammation & Pain
This Anti-Calcium Channel Cav3.1 Antibody is validated for use in WB for the detection of Calcium Channel Cav3.1.
Western blotting: 1:200 using ECL on rat brain membranes. See suggested protocol.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
外形
Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.05% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
储存及稳定性
Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
分析说明
Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Bohumila Jurkovicova-Tarabova et al.
Channels (Austin, Tex.), 12(1), 378-387 (2018-11-08)
Contributions of voltage sensing S4 segments in domains I - IV of CaV3.1 channel to channel activation were analyzed. Neutralization of the uppermost charge in individual S4 segments by exchange of arginine for cysteine was employed. Mutant channels with single
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