AB5252P
Anti-Glial Derived Neurotrophic Factor Antibody
Chemicon®, from sheep
别名:
GDNF
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunohistochemistry
Species reactivity:
human, rat, mouse
Citations:
1
Technique(s):
immunohistochemistry: suitable
Uniprot accession no.:
产品名称
Anti-Glial Derived Neurotrophic Factor Antibody, Chemicon®, from sheep
biological source
sheep
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GDNF(2668)
Application
Detect Glial Derived Neurotrophic Factor using this Anti-Glial Derived Neurotrophic Factor Antibody validated for use in IH.
Immunohistochemistry: 1 μg/mL
Immunoblotting: 1 μg/mL using TBS (not PBS) and blocking is best performed with IgG free BSA highest quality. Antibody recognizes ~75 kDa band in non-acid-treated serum samples corresponding to the GDNF/GDNFR complex. When samples are acid treated, a prominent 32 kDa (dimer) and weaker 16 kDa monomer are observed. Acid treatment: homogenized tissue samples in PBS are acid-treated by adding 1N HCL until the pH is less than 3.0 (pH paper strips), incubated 15 minutes at room temperature and then neutralized by adding 1N NaOH to pH 7.6.
Optimal working dilutions must be determined by end user.
Immunoblotting: 1 μg/mL using TBS (not PBS) and blocking is best performed with IgG free BSA highest quality. Antibody recognizes ~75 kDa band in non-acid-treated serum samples corresponding to the GDNF/GDNFR complex. When samples are acid treated, a prominent 32 kDa (dimer) and weaker 16 kDa monomer are observed. Acid treatment: homogenized tissue samples in PBS are acid-treated by adding 1N HCL until the pH is less than 3.0 (pH paper strips), incubated 15 minutes at room temperature and then neutralized by adding 1N NaOH to pH 7.6.
Optimal working dilutions must be determined by end user.
Physical form
Affinity purified immunoglobulin. Lyophilized. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any residue. Contains no preservative. Glycerol (1:1) can be added for additional stability.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
存储类别
11 - Combustible Solids
wgk
WGK 3
Saurabh Khasnavis et al.
The Journal of biological chemistry, 288(29), 20843-20855 (2013-06-08)
Although Parkinson disease (PD) is a progressive neurodegenerative disorder, available animal models do not exhibit irreversible neurodegeneration, and this is a major obstacle in finding out an effective drug against this disease. Here we delineate a new irreversible model to
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