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AB3623

抗胱天蛋白酶3抗体，活性（裂解）形式

Chemicon®, from rabbit

别名:

Apopain, Yama Protein, CASP3, SCA-1, procaspase3

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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产品名称

抗胱天蛋白酶3抗体，活性（裂解）形式, Chemicon®, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, human

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NCBI accession no.

NM_004346.3

UniProt accession no.

P42574

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... CASP3(836)

Application

抗胱天蛋白酶3抗体（活性（裂解）形式）可检测胱天蛋白酶3的水平，已发表并经验证可用于免疫荧光（IF）、免疫组化（IHC）和蛋白质印迹（WB）。
研究子类别
胱天蛋白酶

酶&生物化学
蛋白质印迹分析：
我们用活性凋亡细胞的1：100-1：200（0.5-4µg/ml）稀释液来检测胱天蛋白酶-3。
注意当使用组织时，活性胱天蛋白酶3的水平可以低于可检测的水平。使用CC119（人胱天蛋白酶3）与1 mM ATP培养30分钟（用于激活），作为westerns上的阳性对照。或者，用星形孢菌素（0.5µM，10-18小时）培养的神经元培养制剂通常会产生很强的胱天蛋白酶3信号。

免疫组化：
1：10稀释度（10-20µg/ml），AB3623在4%PFA固定、石蜡包埋的小鼠组织中反应成功。

免疫组化（石蜡）：
胱天蛋白酶-3单克隆抗体的最佳染色： 小鼠小肠

Biochem/physiol Actions

AB3623仅检测裂解的p17片段，而不检测经历凋亡的细胞中的前体形式。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

17kda
胱天蛋白酶-3（CPP32，Apopain）是研究最广泛的凋亡蛋白。胱天蛋白酶-3被合成为一种无活性的酶原（32 kDa），该酶在经历凋亡的细胞中通过自身蛋白水解而处理和/或被另一种上游蛋白酶切割而处理。胱天蛋白酶-3的加工形式由大（17 kDa）和小（12 kDa）亚基组成，它们结合形成活性酶。活性胱天蛋白酶-3蛋白水解切割并激活其他胱天蛋白酶，以及细胞中的相关靶标，例如PARP和DFF。

Immunogen

表位：活性（裂解）形式

Other Notes

替代品：04-440；04-1088
浓度：请参考批次特异性浓缩物的分析证书。

Physical form

在含有PBS，30%甘油，0.5%BSA，5 mM EDTA和0.03%proclin的缓冲液中纯化的兔多克隆抗体。

Preparation Note

自收到之日起在-20ºC可稳定保存1年。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

hcodes

H317,H411

Hazard Classifications

Aquatic Chronic 2 - Skin Sens. 1

存储类别

10 - Combustible liquids

wgk

WGK 3

分析证书(COA)

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Hypoxic neuronal necrosis: protein synthesis-independent activation of a cell death program.
Niquet, J; Baldwin, RA; Allen, SG; Fujikawa, DG; Wasterlain, CG
Proceedings of the National Academy of Sciences of the USA null
Joseph E Druso et al.
The Journal of biological chemistry, 291(17), 8886-8895 (2016-02-26)
The Rho family small GTPase Cdc42 has been implicated in a wide range of cellular functions including the establishment of cell polarity and the remodeling of the actin cytoskeletal architecture, resulting in the tight regulation of cell growth and survival
C Lv et al.
Cell death & disease, 4, e952-e952 (2013-12-07)
Chemotherapy remains the common therapeutic for patients with lung cancer. Novel, selective antitumor agents are pressingly needed. This study is the first to investigate a different, however, effective antitumor drug candidate Wentilactone A (WA) for its development as a novel
Astrocyte proliferation following stroke in the mouse depends on distance from the infarct.
Barreto, GE; Sun, X; Xu, L; Giffard, RG
Testing null
Kata Osz et al.
Reproductive biology and endocrinology : RB&E, 12, 21-21 (2014-03-19)
Ovarian angiogenesis is a complex process that is regulated by a balance between pro- and anti-angiogenic factors. Physiological processes within the ovary, such as folliculogenesis, ovulation, and luteal formation are dependent upon adequate vascularization and anything that disrupts normal angiogenic
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