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The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

For important updates on recent policy changes, please click here for more details.

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所有图片(4)

主要文件

AB3201

Sigma-Aldrich

Anti-β-Tubulin III Antibody

CHEMICON®, rabbit polyclonal

别名:

Glu Tubulin

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

如需咨询该产品AB3201,请联系默克当地办事处或经销商。 联系客户支持

产品名称

抗微管蛋白抗体,去酪氨酸化, Chemicon®, from rabbit

生物来源

rabbit

质量水平

抗体形式

affinity purified immunoglobulin

抗体产品类型

primary antibodies

克隆

polyclonal

纯化方式

affinity chromatography

种属反应性

rat, mouse, sea urchin, human, plant

请勿与下列物质发生反应

Saccharomyces cerevisiae

种属反应性(根据同源性预测)

mammals

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此商品
EP0030000765EP0030000951EP0030000978
sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

material

turquoise

material

turquoise

material

red

material

-

packaging

pack of 200 ea (2 bags × 100 tips)

packaging

pack of 200 ea (2 bags × 100 tips)

packaging

pack of 500 ea (5 bags × 100 tips)

packaging

pack of 500 ea (5 bags × 100 tips)

availability

available only in (India)

availability

available only in (India)

availability

available only in (India)

availability

available only in (India)

capacity

1-10 mL

capacity

0.5-10 mL

capacity

500-2500 μL

capacity

100-5000 μL

一般描述

微管参与多种细胞活动,从有丝分裂和转运事件到细胞运动和细胞形状的维持。微管蛋白本身是一种球状蛋白,由两种多肽(α和β微管蛋白)组成。将α和β微管蛋白二聚体组装到13个原丝上,形成直径为22 nm的微管。酪氨酸连接酶将C端酪氨酸释放到单体α微管蛋白上。组装的微管可以再次被细胞骨架相关的羧肽酶脱酪氨酸。脱酪氨酸化的α微管蛋白称为Glu-微管蛋白。脱酪氨酸化的α微管蛋白的另一种翻译后修饰是C末端聚谷氨酰化,它是神经元细胞和有丝分裂纺锤体中微管的特征。

特异性

该抗体可特异性识别微管蛋白α链的脱酪氨酸形式(Glu微管蛋白)(Bianchi, M., 2006; Goulimari, P., 2005).
预期与哺乳动物、海胆、植物发生交叉反应,而不与酵母发生交叉反应(Paturle-Lafanechere, L., 1991)。

免疫原

对应于Glu微管蛋白C末端的肽。

应用

免疫组化:
先前批次用于冷冻或石蜡包埋的固定组织(Gundersen, G.G., 1984):1:200-1:500稀释液。

最佳工作稀释度必须由最终用户确定。
去酪氨酸化抗微管蛋白抗体是用于IC、IH(P)和WB的针对微管蛋白的抗体。
研究子类别
细胞骨架信号转导
研究类别
细胞结构

质量

通过蛋白质印迹对L6裂解物进行了常规评估。

蛋白质印迹分析: 该批次的1:500稀释液在10μg L6裂解物中检测到去酪氨酸化微管蛋白。

目标描述

50kda

外形

亲和纯化的免疫球蛋白
在含有液体PBS的缓冲液中纯化的兔多克隆抗体。

储存及稳定性

自收到之日起,以未稀释的等分试样可于-20°C下稳定保存6个月。

使用建议:
第一次融化后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。 分装到微量离心管中,并储存于 -20°C。 避免反复冻融循环,以免损坏 IgG 和影响产品性能。

分析说明

对照
HeLa全细胞裂解物。

其他说明

浓度:请参考批次特异性浓缩物的分析证书。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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在文件库中查找您最近购买产品的文档。

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Carolyn Ott et al.
Cilia, 1(1), 3-3 (2013-01-29)
The central tenet of cilia function is sensing and transmitting information. The capacity to directly contact extracellular surfaces would empower primary cilia to probe the environment for information about the nature and location of nearby surfaces. It has been well
Chunmei Li et al.
PLoS biology, 14(3), e1002416-e1002416 (2016-03-18)
Cilia have a unique diffusion barrier ("gate") within their proximal region, termed transition zone (TZ), that compartmentalises signalling proteins within the organelle. The TZ is known to harbour two functional modules/complexes (Meckel syndrome [MKS] and Nephronophthisis [NPHP]) defined by genetic
Joanna Lipka et al.
The EMBO journal, 35(3), 302-318 (2016-01-14)
In neurons, the polarized distribution of vesicles and other cellular materials is established through molecular motors that steer selective transport between axons and dendrites. It is currently unclear whether interactions between kinesin motors and microtubule-binding proteins can steer polarized transport.
Daehwan Kim et al.
Oncotarget, 7(14), 17829-17843 (2016-02-20)
Mammalian diaphanous-related formin 1 (mDia1) expression has been linked with progression of malignant cancers in various tissues. However, the precise molecular mechanism underlying mDia1-mediated invasion in cancer cells has not been fully elucidated. In this study, we found that mDia1
Yoshiaki V Nishimura et al.
Development (Cambridge, England), 141(18), 3540-3550 (2014-09-04)
Neuronal migration is crucial for development of the mammalian-specific six-layered cerebral cortex. Migrating neurons are known to exhibit distinct features; they form a cytoplasmic dilation, a structure specific to migrating neurons, at the proximal region of the leading process, followed

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