Anti-β-Actin (ACTB) Antibody

During apoptosis, beta actin is cleaved by activated caspase 3 between Asp244 and Gly245. This cleavage results in the release of an approximately 130 amino acid (15 kDa) fragment from the C-terminus of beta actin and a 32 kDa (244 amino acid) N-terminal fragment of actin, known as Fractin. The C-terminus of the Fractin fragment represents an apoptosis-specific neoepitope that is not antigenic in native full-length beta actin and therefore presents a novel and specific marker for distinguishing apoptotic from necrotic cells.

By immunohistochemistry and Western blot, this antibody only recognizes the 32 kDa Fractin fragment but not full-length beta actin.

K-YELPD peptide representing the last five amino acids of the C-terminus of the 32 kDa Fractin fragment of beta-Actin. This sequence is absolutely conserved in Fractin fragments from human, mouse, rat, feline, porcine, ovine, bovine, canine, rabbit and guinea pig origin.

Detect Fractin using this Anti-Fractin Antibody, C-terminus validated for use in WB, IC, IH(P).

Immunohistochemistry/Immunocytochemistry: 1:400-1:1000. 1 hr at 37°C or 4°C overnight. Note: If studying paraffin-embedded tissue, the use of antigen retrieval methods is recommended.

Western Blot: 1:1000-1:4000 for incubation overnight at 4°C. Recommended antibody dilution buffer: TBS with 3% BSA and 0.05% Tween 20, pH 7.4

Optimal working dilutions must be determined by end user.

Liquid whole rabbit serum containing no preservatives.

Aliquot and store at -20°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles.

Control
POSITIVE CONTROL: Staurosporin treated neuronal cultures.

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