生物来源
rabbit
质量水平
抗体形式
affinity purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
mouse
制造商/商品名称
Chemicon®
技术
ELISA: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
NCBI登记号
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
mouse ... Lama1(16772)
特异性
从小鼠肿瘤组织中提取并纯化的层粘连蛋白。 抗体与I、II和IV型小鼠胶原蛋白的反应性低于0.1%;I、III和V型大鼠胶原蛋白;大鼠和人弹性蛋白;放射免疫法测定小鼠纤维连接蛋白和层粘连蛋白。
免疫原
从EHC小鼠肿瘤组织中提取并纯化的层粘连蛋白。
应用
IFA(与荧光素抗兔IgG偶联物)-在冷冻小鼠组织(皮肤、肝脏等)上以≥1:80稀释。
IHC(石蜡)-在固定、石蜡包埋的小鼠组织(皮肤、肝脏等)上以≥1:500稀释使用。(使用Envision/Dako试剂盒的免疫过氧化物酶技术);
ELISA-以≥1:200稀释使用(OD ≥ 500)。
最佳工作稀释度必须由最终用户进行确定。
IHC(石蜡)-在固定、石蜡包埋的小鼠组织(皮肤、肝脏等)上以≥1:500稀释使用。(使用Envision/Dako试剂盒的免疫过氧化物酶技术);
ELISA-以≥1:200稀释使用(OD ≥ 500)。
最佳工作稀释度必须由最终用户进行确定。
抗层粘连蛋白抗体可检测层粘连蛋白水平 & 经发布 & 验证可用于ELISA、RIA、IH。
研究子类别
ECM 蛋白
ECM 蛋白
研究类别
细胞结构
细胞结构
外形
纯化抗体溶于缓冲液中,内含0.1M柠檬酸盐、0.1M磷酸钾盐(pH 7.2-7.4)和10 µg/ml抗生素和抗真菌剂
储存及稳定性
自收到之日起,在-20°C下可保持冷冻长达12个月。 应避免反复冻/融循环。
其他说明
浓度:请参考批次特异性浓缩物的检验报告。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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