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Merck
CN

AB19012

Anti-Anti-Laminin (LAMA1) antibody

CHEMICON®, rabbit polyclonal

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关于此项目

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
human
Application:
ELISA, IHC, IP, WB
Citations:
34
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产品名称

抗层粘连蛋白抗体, Chemicon®, from rabbit

biological source

rabbit

antibody form

affinity purified immunoglobulin

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

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Immunogen

全长人层粘连蛋白。 通过SDS-PAGE评估单带纯度后发生免疫。

Application

使用经验证可用于 ELISA、IP、WB、IH的抗层粘连蛋白抗体检测层粘连蛋白。
免疫印迹: 1:500-1:2000

ELISA: 1:4000-1:8000

免疫组化: 1:50-1:200

免疫沉淀:条件必须由最终用户确定

最佳工作稀释度必须由最终用户进行确定。

Biochem/physiol Actions

该产品是通过使用固定化抗原的免疫亲和层析制备的,然后针对人血清蛋白,胶原蛋白和非胶原细胞外基质蛋白进行广泛的交叉吸附,以去除不需要的反应性。 通常,通过ELISA针对纯化的标准品检测到与其他细胞外基质蛋白的交叉反应性低于1%。 该抗体与大多数哺乳动物层粘连蛋白反应,与I、II、III、IV、V或VI型胶原或纤维连接蛋白的交叉反应性可忽略不计。 抗层粘连蛋白抗体与其他人血清蛋白或非层粘连蛋白细胞外基质蛋白的非特异性交叉反应可忽略不计。

Physical form

亲和纯化的免疫球蛋白。 1 mg/mL液体,溶于0.125 M硼酸钠、0.075 M氯化钠、0.005 M EDTA(pH 8.0)和0.01%叠氮化钠中。
形式:纯化

Preparation Note

未稀释的抗体溶液在2-8° C下可稳定储存约6个月。对于延长储存期,与等体积甘油混合,等分并储存在-20°C下。 应避免反复冻/融循环。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Repr. 1B

存储类别

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Dhruv Sareen et al.
PloS one, 4(10), e7630-e7630 (2009-11-10)
Stem cell expansion and differentiation is the foundation of emerging cell therapy technologies. The potential applications of human neural progenitor cells (hNPCs) are wide ranging, but a normal cytogenetic profile is important to avoid the risk of tumor formation in
MMP-14 and TIMP-2 overexpression protects against hydroquinone-induced oxidant injury in RPE: implications for extracellular matrix turnover.
Alcazar, O; Cousins, SW; Marin-Casta?o, ME
Investigative Ophthalmology & Visual Science null
Yuichiro Higuchi et al.
Journal of cell science, 123(Pt 16), 2733-2742 (2010-07-22)
We previously reported that embryonic stem (ES) cells cultured on M15 cells, a mesoderm-derived supportive cell line, were efficiently differentiated towards an endodermal fate, finally adopting the specific lineages of various digestive organs such as the pancreas and liver. We
Enamel matrix proteins bind to wound matrix proteins and regulate their cell-adhesive properties.
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European Journal of Oral Sciences null
Cathepsin-L influences the expression of extracellular matrix in lymphoid organs and plays a role in the regulation of thymic output and of peripheral T cell number.
Gabriela Lombardi, Dalia Burzyn, Juliana Mundi?ano, Paula Berguer, Pedro Bekinschtein et al.
Journal of immunology (Baltimore, Md. : 1950) (1950)

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