AB1589P
Anti-Adenosine A2b Receptor Antibody
Chemicon®, from rabbit
别名:
A2b Receptor Antibody, Adenosine A2b Antibody, Anti-Adenosine Receptor
产品名称
Anti-Adenosine A2b Receptor Antibody, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ADORA2B(136)
Application
Detect Adenosine A2b Receptor using this Anti-Adenosine A2b Receptor Antibody validated for use in ELISA, IC, IH & WB.
Immunohistochemistry: 10-50 μg/mL on frozen and formalin fixed tissue sections. The receptor was localized in epithelial cells of the crypts, and in synctiotrophoplast cells of human placenta and in T84 human colon adenocarcinoma cells.
Western blot: 1-10 μg/mL (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.
Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.
ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG292)/well)
Optimal working dilutions must be determined by end user.
Western blot: 1-10 μg/mL (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.
Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.
ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG292)/well)
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Circadian Rhythm & Sleep
Neurotransmitters & Receptors
Circadian Rhythm & Sleep
Biochem/physiol Actions
Adenosine A2b receptor. Recognizes a band of approximately 50-52 kDa in several rat, mouse and human tissues (thymus, placenta, colon and small intestine). The antibody also recognizes the 35 kDa band in small intestine. No bands were detected in skeletal muscle or heart.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Amino acid sequence corresponding to the 2nd extracellular domain (16 amino acids) of the human brain A2bR gene (Pierce et al. 1992; Tucker & Linden 1993).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Tadeusz Pawelczyk et al.
The American journal of pathology, 167(2), 315-325 (2005-07-29)
Pathological alterations of renal function in insulin-dependent diabetes have been attributed to numerous factors, including adenosine. This study examined the expression levels of adenosine receptors (ARs) in the kidney of the streptozotocin-induced diabetic rat. In the diabetic kidney A1-AR mRNA
Shefeeq M Theparambil et al.
Nature, 632(8023), 139-146 (2024-07-04)
Brain computation performed by billions of nerve cells relies on a sufficient and uninterrupted nutrient and oxygen supply1,2. Astrocytes, the ubiquitous glial neighbours of neurons, govern brain glucose uptake and metabolism3,4, but the exact mechanisms of metabolic coupling between neurons
Elizabeth Salvo et al.
Heliyon, 5(2), e01223-e01223 (2019-03-01)
Pain associated with oral squamous cell carcinoma (oral SCC) decreases quality of life and survival. The interaction between cancer and the peripheral nerves is known to initiate and amplify pain and contribute to carcinogenesis. Schwann cells envelop peripheral nerves and
Zuokang Zheng et al.
Cell death & disease, 9(11), 1133-1133 (2018-11-16)
MicroRNAs are a group of small non-coding RNAs that play key roles in almost every aspect of mammalian cell. In kidney, microRNAs are required for maintaining normal function of renal cells, disruption of which contributes to pathogenesis of renal diseases.
Alejandro Sánchez-Melgar et al.
Nutrients, 13(9) (2021-09-29)
Neurodegenerative disorders are devastating diseases in which aging is a major risk factor. High-fat diet (HFD) seems to contribute to cognition deterioration, but the underlying mechanisms are poorly understood. Moreover, resveratrol (RSV) has been reported to counteract the loss of
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