Anti-NMDAR2A&B Antibody, pan

Western blot analysis of transfected cells shows the antibody recognizes both NR2A and NR2B subunits equally. There may be a very slight recognition of NR2C and NR2D. Western blot analysis of rat brain shows a single band comigrating with NMDAR2A and NMDAR2B expressed in transfected cells. AB1548 has also been used effectively in Western blot of the membrane fraction of rabbit cortex and avian brain homogenates. No cross-reaction is seen with NMDAR1 or other glutamate receptor subunits.

Synthetic peptide (LNSCSNRRVYKKMPSIESDV) corresponding to the C-terminus of rat NMDAR2A receptor subunit conjugated to BSA.

Anti-NMDAR2A&B Antibody, pan detects level of NMDAR2A&B & has been published & validated for use in IH(P), IC, IH, IP & WB.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Western blot analysis can be done at final concentrations of 0.25-0.5 μg/mL; recognizes a single band in membrane preparations of rat hippocampal neurons ~170,000-180,000 under reduced, SDS PAGE. AB1548 can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations recommended are 0.5-1.0 μg/mL.

The antibody can be used for immunopreciptation of detergent solubilized receptor from brain or transfected cells. For most cases 5 μg of antibody is used with immobilized Protein A.

Optimal working dilutions must be determined by end user.

Affinity purified using the immunogen peptide. Lyophilized from PBS containing 1% BSA.



Note: lot 0509010940 was shipped in liquid format; Do NOT add additional water to this lot of material.

Maintain lyophilized material at 2-8°C or -20°C. Reconstitute to 500 μL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Avoid repeated thawing and refreezing. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks and reused repeatedly.

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