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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
mouse, human, feline, bat, rat, monkey
Application:
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
immunoprecipitation (IP)
western blot
immunoprecipitation (IP)
western blot
Technique(s):
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Citations:
179
Uniprot accession no.:
产品名称
抗胆碱乙酰基转移酶(ChAT)抗体, serum, Chemicon®
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human, feline, bat, rat, monkey
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
cat ... Chat(751512)
human ... CHAT(1103)
mouse ... Chat(12647)
rat ... Chat(290567)
rhesus monkey ... Chat(709977)
Biochem/physiol Actions
与其他物种的反应性尚未通过IHC得到确认。
大脑和中枢神经紧张的系统中的胆碱能神经元。
Analysis Note
对照
大脑皮层组织。
大脑皮层组织。
通过免疫组化(石蜡)对正常大脑皮层组织进行常规评估。
免疫组织化学(石蜡)分析:
正常大脑皮层ChAT(目录#AB143)染色。未经抗原修复处理的组织。使用带有HRP-DAB的IHC-Select Detection,将抗体稀释至1:100。 免疫反应性被视为神经元(棕色)的细胞体、树突和轴突上的染色模式。
免疫组织化学(石蜡)分析:
正常大脑皮层ChAT(目录#AB143)染色。未经抗原修复处理的组织。使用带有HRP-DAB的IHC-Select Detection,将抗体稀释至1:100。 免疫反应性被视为神经元(棕色)的细胞体、树突和轴突上的染色模式。
Application
免疫沉淀:
先前批次的1:200稀释液可从大脑和胎盘中免疫沉淀68 kDa活性ChAT。还会在人类和其他哺乳动物的中枢神经紧张系统的某些胆碱能神经元中沉淀出与ChAT共表达(但缺乏ChAT活性)的27 kDa蛋白。
最佳的工作稀释度和方案必须由最终用户确定。
先前批次的1:200稀释液可从大脑和胎盘中免疫沉淀68 kDa活性ChAT。还会在人类和其他哺乳动物的中枢神经紧张系统的某些胆碱能神经元中沉淀出与ChAT共表达(但缺乏ChAT活性)的27 kDa蛋白。
最佳的工作稀释度和方案必须由最终用户确定。
抗胆碱乙酰基转移酶(ChAT)抗体是一种抗胆碱乙酰基转移酶(ChAT)的抗体,用于IH、IH(P)、IP & WB,产品引用超过70次。
General description
乙酰胆碱(ACh)是运动神经元、神经节前自主神经元、神经节后副交感神经元、各种大脑区域和一些新兴的神经元样干细胞的常见神经递质。Ach 的代谢相对简单,仅涉及两种酶:用于合成的胆碱乙酰基转移酶(ChAT)和用于降解的乙酰胆碱酯酶(AChE)。此外,乙酰胆碱在除神经传递以外的神经元中几乎没有功能,并且似乎是神经元特异性的。似乎只有胆碱能神经元具有大量的ChAT,使得抗胆碱乙酰基转移酶成为有用的特异性标志物。
Other Notes
浓度:请参考批次特异性浓缩物的检验报告。
Physical form
兔多克隆抗血清,液体,不含防腐剂。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Silencing of Cholinergic Basal Forebrain Neurons Using Archaerhodopsin Prolongs Slow-Wave Sleep in Mice.
Shi, YF; Han, Y; Su, YT; Yang, JH; Yu, YQ
Testing null
Nancy Lee et al.
The Journal of comparative neurology, 525(5), 1206-1215 (2016-11-05)
Exogenous ciliary neurotrophic factor (CNTF) administration promotes the survival of motor neurons in a wide range of models. It also increases the expression of the critical neurotransmitter enzyme choline acetyltransferase (ChAT) by in vitro motor neurons, likely independent of its
Hypocretin1/OrexinA axon targeting of laterodorsal tegmental nucleus neurons projecting to the rat medial prefrontal cortex.
Cid-Pellitero, Ed; Garzon, M
Cerebral Cortex (1991)
Marc R Kamke et al.
Neuron, 48(4), 675-686 (2005-11-23)
The putative role of the basal forebrain cholinergic system in mediating lesion-induced plasticity in topographic cortical representations was investigated. Cholinergic immunolesions were combined with unilateral restricted cochlear lesions in adult cats, demonstrating the consequence of cholinergic depletion on lesion-induced plasticity
S W Wang et al.
Genes & development, 15(1), 24-29 (2001-01-13)
math5 is a murine orthologue of atonal, a bHLH proneural gene essential for the formation of photoreceptors and chordotonal organs in Drosophila. The expression of math5 coincides with the onset of retinal ganglion cell (RGC) differentiation. Targeted deletion of math5
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