推荐产品
生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
human, pig
种属反应性(根据同源性预测)
mammals, rat
制造商/商品名称
Chemicon®
技术
immunohistochemistry: suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... CYP3A4(1576)
特异性
兔多克隆血清检测人和大鼠体内CYP3A4;通过所用免疫原的序列同源性,可以预期与CYP3A7、3A6V4;3A2V1、3A8、3A9的交叉反应性,但此时尚未测试。
免疫原
含有来自人CYP3A4的TVSGA的C端序列的合成肽。该肽与KLH偶联。
应用
抗细胞色素P450酶CYP3A4抗体可检测细胞色素P450酶CYP3A4水平 & 已出版 & 经过验证可用于IH & WB。
研究子类别
酶&生物化学
酶&生物化学
研究类别
代谢
代谢
蛋白质印迹: 1:1000-1:2000:使用微粒体膜级分,检测~54 kDa的条带;也可能存在其他条带。 抗体已成功用于LICOR荧光westerns检测CYP3A4。
免疫组化:10%福尔马林固定,石蜡包埋的组织1:200-1:400,建议使用微波-柠檬酸抗原回收。
最佳工作稀释度必须由最终用户确定。
免疫组化:10%福尔马林固定,石蜡包埋的组织1:200-1:400,建议使用微波-柠檬酸抗原回收。
最佳工作稀释度必须由最终用户确定。
目标描述
57 kDa
外形
兔抗血清。 不含防腐剂的液体。
未纯化
储存及稳定性
自收到之日起在-20°C可稳定保存1年。避免反复冻融。为了最大程度地回收产品,在融化后和取下盖子之前,将原始小瓶进行离心。
分析说明
对照
肝组织
肝组织
其他说明
浓度:请参考批次特异性浓缩物的检验报告。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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The circadian rhythm has profound effect on the body, exerting effects on diverse events like sleep-wake patterns, eating behavior and hepatic detoxification. The cytochrome p450 s (Cyps) is the main group of enzymes responsible for detoxification. However, the underlying mechanisms
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Endogenous long-chain metabolites of vitamin E (LCMs) mediate immune functions by targeting 5-lipoxygenase (5-LOX) and increasing the systemic concentrations of resolvin E3, a specialized proresolving lipid mediator. SAR studies on semisynthesized analogues highlight α-amplexichromanol (27a), which allosterically inhibits 5-LOX, being
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Despite the increasing use of the minipig as a non-rodent species in general and juvenile toxicity studies, knowledge on their biotransformation processes and their ontogeny is scarce. Such data are prerequisite for the correct interpretation of non-clinical studies in this
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Endogenous TRPV1 agonists such as oxidized linoleic acid metabolites (OLAMs) and the enzymes releasing them [eg, cytochrome P450 (CYP)] are up-regulated after inflammation in the rat. However, it is not known whether such agonists are elevated in human inflammatory pain
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