推荐产品
形式
liquid
制造商/商品名称
Novagen®
储存条件
OK to freeze
运输
wet ice
一般描述
Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis
YeastBuster<TMSYMBOL></TMSYMBOL> Protein Extraction Reagent is formulated for a fast, efficient and gentle extraction of active proteins from yeast cells. The reagent avoids harsh conditions of vigorous mechanical or chemical treatment that often result in heat degradation of target proteins. The proprietary formulation utilizes a mix of mild detergent, protein stabilization buffer, and tris(hydroxypropyl) phosphine (THP) reducing agent (THP concentrate provided separately). This powerful combination eliminates the inconsistencies associated with tedious mechanical disruption of yeast cells with glass bead abrasives, ultrasonication and pressure disruption, or enzymatic digestion with β-1,3-glucanase lytic enzymes. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, and Schizosaccharomyces pombe strains and with plant cells.
In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind<TMSYMBOL></TMSYMBOL> and Ni-NTA HIS-BIND® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.
In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind<TMSYMBOL></TMSYMBOL> and Ni-NTA HIS-BIND® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.
特点和优势
- Gentle, rapid, efficient extraction of proteins from yeast cells
- Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
- Higher yield of total and enzymatically active proteins
- Fully compatible with Ni-NTA His•Bind and GST•Bind affinity purification methods
组分
•100 ml or 500 mlYeastBuster Protein Extraction Reagent
•1 ml or 5 ml100X THP Solution
•1 ml or 5 ml100X THP Solution
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
其他说明
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
法律信息
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Warning
危险声明
预防措施声明
危险分类
STOT RE 2 Oral
靶器官
Kidney
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°C)
Not applicable
Mark Zander et al.
Nature plants, 6(3), 290-302 (2020-03-15)
Understanding the systems-level actions of transcriptional responses to hormones provides insight into how the genome is reprogrammed in response to environmental stimuli. Here, we investigated the signalling pathway of the hormone jasmonic acid (JA), which controls a plethora of critically
商品
YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast
相关内容
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
细胞裂解和蛋白质溶解是进行有效分析和高效处理的关键步骤，默克分享提取方法包括酶法、化学法、机械法或几种方法的联合使用。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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