等级
Molecular Biology
形式
liquid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
异质活性
DNase, RNase, protease, none detected
运输
ambient
储存温度
15-25°C
一般描述
10X 浓缩物,可稀释成2X溶液,其中含有89 mM Tris、89 mM醋酸盐和2 mM EDTA,pH~8.3。
三硼酸盐和乙二胺四乙酸(TBE)缓冲液用于DNA和RNA的琼脂糖凝胶电泳。它由一种以中性和阴离子形式存在的弱酸组成(比如,COOH和COO- 物种)。Tris是一种弱碱,以中性或阳离子形式存在(Tris-NH2 和Tris-NH3+)。这些离子保持介质的低导电性,可传输电流以及缓冲pH值。乙二胺四乙酸(EDTA)虽然并非必需,但可用作预防添加,因为它螯合Mg2+离子并使潜在的DNA核酸酶失活。TBE允许DNA片段的有效分辨率,并略微改善较小片段的分离。它还能稳定核酸,防止酶降解。
应用
TBE缓冲液(10倍),分子生物学级 - Calbiochem已用为聚丙烯酰胺测序凝胶的组分,用于通过扩增片段长度多态性(AFLP)进行DNA电泳。
警告
毒性:刺激性(B)
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语:
Danger
危险声明
危险分类
Repr. 1B
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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Optical properties of buffers and cell culture media for optofluidic and sensing applications
Applied Sciences, 9(6), 1145-1145 (2019)
Analytical biochemistry, 454, 44-52 (2014-03-19)
Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric
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