516560
Pertussis toxin
from Bordetella pertussis, Five distinct bands by SDS-PAGE, lyophilized, protein endotoxin, Calbiochem
别名:
百日咳毒素，百日咳杆菌
产品名称
百日咳毒素，无盐，百日咳杆菌, This Pertussis toxin, CAS 70323-44-3, catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and Gt.
SMILES string
O(C)C(=O)CCCCCCCCCCCCCCCCCCCCCCCC
InChI
1S/C26H52O2/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20-21-22-23-24-25-26(27)28-2/h3-25H2,1-2H3
InChI key
WOPKHAQDUMDJIY-UHFFFAOYSA-N
description
RTECS - XW5883750
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
solubility
soluble (Reconstitute with 500 µl H2O. Obtain a uniform suspension before each use by scraping the walls of the tube and gently pipetting the solution to mix.)
foreign activity
adenylate cyclase ≤35 pmol/min-μg (in the presence of calmodulin)
shipped in
ambient
storage temp.
2-8°C
Quality Level
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Analysis Note
SDS-PAGE法测得5个不同条带
Application
来自百日咳杆菌的百日咳毒素已被用作Gi蛋白抑制剂，用于研究百日咳毒素减少下丘脑阿丙鸦片黑素皮质素（POMC）神经元去极化反应的能力。在百日咳毒素实验中，它还可用于阻断Gi，进行体内内耳和体外外毛细胞（HC）研究。
Biochem/physiol Actions
主要靶标
鸟嘌呤核苷酸结合调节蛋白Gi，Go和Gt
鸟嘌呤核苷酸结合调节蛋白Gi，Go和Gt
产物不与ATP竞争。
Disclaimer
毒性：有毒（F）
General description
百日咳毒素（PT）是百日咳杆菌分泌的蛋白外毒素和致病因子，是百日咳的病原体。它通过由ptl基因编码的IV型分泌系统穿过细菌外膜进行运输，ptl基因位于编码毒素的ptx基因的下游。这种具有AB5结构的复杂多亚单位毒素是非细胞百日咳疫苗的关键成分。作为单组分类毒素它可以发挥保护作用。此外，由于其对G蛋白偶联受体途径的抑制作用，它被广泛应用于哺乳动物细胞信号传导研究。它抑制中性粒细胞募集和气道内流（airway influx），这是对细菌感染的常见炎症反应。
Other Notes
Joshi, S.A., et al. 1998.FEBS Lett. 441, 67.
Fryer, M.W.1992.Neurosci. Lett.146, 84.
Gierschik, P. 1992.Curr.Topics Microbiol.Immunol.175, 69.
Harada, J., et al. 1992.Eur. J. Pharmacol.227, 301.
Hausman, S.Z., and Burns, D.L.1992.J. Biol. Chem.267, 13735.
Kaslow, H.R., and Burns, D.L., 1992.FASEB J.6, 2684.
Zhu, J., et al. 1992.J. Pharmacol.Exp.Therap.263, 1479.
Hewlett, E.L., et al. 1983.Infect.Immun.40, 1198.
Moss, J., et al. 1983.J. Biol. Chem.258, 11879.
Fryer, M.W.1992.Neurosci. Lett.146, 84.
Gierschik, P. 1992.Curr.Topics Microbiol.Immunol.175, 69.
Harada, J., et al. 1992.Eur. J. Pharmacol.227, 301.
Hausman, S.Z., and Burns, D.L.1992.J. Biol. Chem.267, 13735.
Kaslow, H.R., and Burns, D.L., 1992.FASEB J.6, 2684.
Zhu, J., et al. 1992.J. Pharmacol.Exp.Therap.263, 1479.
Hewlett, E.L., et al. 1983.Infect.Immun.40, 1198.
Moss, J., et al. 1983.J. Biol. Chem.258, 11879.
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Physical form
在50 mM NaCl，10 mM磷酸钠缓冲液，pH 7.0中冻干。
Preparation Note
在复溶后，冷藏（4°C）。为了在溶液中长期保存，请使用高离子强度缓冲液（例如100 mM磷酸钠，500 mM氯化钠，pH值7.0）复溶。贮备液在CHO细胞试验中可稳定至少6个月，在非细胞系统中可稳定1-2个月。
用500µl H₂O复溶。每次使用前，刮擦管壁并轻轻移取溶液混合，以获得均匀的混悬剂。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
高风险级别生物产品-Merck
此项目有
Pertussis Toxin: Structure-Function Relationship
Locht C, et al.
Bacterial Protein Toxins, 167-185 (2000)
Live imaging of hair bundle polarity acquisition demonstrates a critical timeline for transcription factor Emx2
Tona Y and Wu DK
eLife, 9, e59282-e59282 (2020)
Nicholas H Carbonetti
Future microbiology, 5(3), 455-469 (2010-03-10)
Pertussis toxin and adenylate cyclase toxin are two important virulence factors of Bordetella pertussis, the bacterial cause of the respiratory disease pertussis or whooping cough. In addition to studies on the structure, function and role in pathogenesis of these two
P Órdenes et al.
Scientific reports, 11(1), 21644-21644 (2021-11-06)
Previous studies indicate that the activity of hypothalamic POMC neurons can be regulated by glucose via intracellular mechanisms, but its regulation by lactate is poorly understood. In addition to its energetic role, lactate acts as a signaling molecule. In this
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