420200
JC-1
A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria.
别名:
JC-1, 5，5ʹ，6，6ʹ四氯-1，1ʹ，3，3ʹ-四乙基苯并咪唑基碳菁碘化物
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关于此项目
经验公式（希尔记法）:
C25H27Cl4IN4
化学文摘社编号:
分子量:
652.23
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.25
产品名称
JC-1, A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria.
SMILES string
[I-].Clc1cc2[n+](c([n](c2cc1Cl)CC)\C=C\C=[c]3[n](c4c([n]3CC)cc(c(c4)Cl)Cl)CC)CC
InChI
1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1
InChI key
FYNNIUVBDKICAX-UHFFFAOYSA-M
assay
≥95% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
red-orange
solubility
DMSO: 5 mg/mL
shipped in
ambient
storage temp.
−20°C
Quality Level
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Biochem/physiol Actions
主要靶标
用作细胞，组织和分离的线粒体中线粒体电位δΨm的比率指示剂
用作细胞，组织和分离的线粒体中线粒体电位δΨm的比率指示剂
产物不与ATP竞争。
可逆：否
细胞渗透性：否
Disclaimer
毒性：刺激性（B）
General description
一种阳离子、荧光、碳菁染料，可用作细胞、组织和分离的线粒体中线粒体电位ΔΨm的比率计指示剂。它用高膜电位橙色标记线粒体，用低膜电位绿色标记线粒体。线粒体去极化由红色/绿色荧光强度比的降低指示。非常适合检测由线粒体呼吸解偶联剂诱导的ΔΨm衰竭。电位敏感的色移是由于红色荧光J聚集体的浓度依赖性形成。JC-1还已用于多药耐药细胞的功能测定和凋亡相关的线粒体修饰。
Other Notes
Mathur, A., et al. 2000.Cardiovasc.Res. 46, 126.
Gravance, C.G., et al. 2000.Theriogenology53, 1691.
Chanda, B., and Mathew, M.K.1999.Biochim Biophys.Acta 1416, 92.
Dedov, V.N., and Roufogalis, B.D.1999.FEBS Lett.456, 171.
Polla, B.S., et al. 1996.Proc.Natl.Acad.Sci. USA 93, 6458.
White, R.J., and Reynolds, I.J.1996.J. Neurosci. 16, 5688.
Ankacrona, M., et al. 1995.Neuron 15, 961.
Reers, M., et al. 1995.Methods Enzymol.260, 406.
Cossarizza, A., et al. 1993.Biochem.Biophys.Res. Commun.197, 40.
Gravance, C.G., et al. 2000.Theriogenology53, 1691.
Chanda, B., and Mathew, M.K.1999.Biochim Biophys.Acta 1416, 92.
Dedov, V.N., and Roufogalis, B.D.1999.FEBS Lett.456, 171.
Polla, B.S., et al. 1996.Proc.Natl.Acad.Sci. USA 93, 6458.
White, R.J., and Reynolds, I.J.1996.J. Neurosci. 16, 5688.
Ankacrona, M., et al. 1995.Neuron 15, 961.
Reers, M., et al. 1995.Methods Enzymol.260, 406.
Cossarizza, A., et al. 1993.Biochem.Biophys.Res. Commun.197, 40.
Packaging
用惰性气体包装
Preparation Note
复溶后，等分并冷冻保存（-20°C）。储备溶液在-20°C下可稳定保存至多6个月。请勿重复使用解冻样品。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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