36-010
Anti-phospho-MKK7/SKK4 (Thr275/Ser277) Antibody
Upstate®, from rabbit
别名:
Dual specificity mitogen-activated protein kinase kinase 7, EC:2.7.12.2, MAP kinase kinase 7, MAPKK 7, JNK-activating kinase 2, MAPK/ERK kinase 7, MEK 7, Stress-activated protein kinase kinase 4, SKK4, SAPK kinase 4, SAPKK-4, SAPKK4, c-Jun N-terminal kin
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所有图片(2)
About This Item
UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
human
种属反应性（根据同源性预测）
rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
制造商/商品名称
Upstate®
技术
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
dry ice
靶向翻译后修饰
phosphorylation (pThr275/pSer277)
基因信息
human ... MAP2K7(5609)
一般描述
Dual specificity mitogen-activated protein kinase kinase 7 (UniProt: O14733; also known as EC:2.7.12.2, MAP kinase kinase 7, MAPKK 7, JNK-activating kinase 2, MAPK/ERK kinase 7, MEK 7;Stress-activated protein kinase kinase 4, SKK4, SAPK kinase 4, SAPKK-4, SAPKK4, c-Jun N-terminal kinase kinase 2,JNK kinase 2, JNKK 2) is encoded by the MAP2K7 (also known as JNKK2, MEK7, MKK7, RKMK7, SKK4) gene (Gene ID: 5609) in human. MKK4/SKK4 belongs to the STE Ser/Thr protein kinase family and is an essential component of the MAP kinase signal transduction pathway. MKK4/SKK4 is ubiquitously expressed with highest level of expression reported in skeletal muscle. It contains a single protein kinase domain and has a DVD domain (aa 377-400) that contains a conserved docking site and bind to their specific upstream MAP kinase kinase kinases (MAPK3Ks). MKK4/SKK4 also has a D domain in the N-terminal region (aa 37-57), which contains a conserved docking site that is required for the binding of MAPK substrates. It is activated by phosphorylation on Ser271 and Thr275 by MAP kinase kinase kinases (MAP3Ks) and it directly phosphorylates and activates the c-Jun NH2-terminal kinases (JNK), in response to cellular stresses and pro-inflammatory cytokines. Mice lacking the MKK4 gene are shown to have abnormal hepatogenesis and die before embryonic day 14.
特异性
This rabbit polyclonal antibody detects MKK7/SKK4 phosphorylated on Thr275/Ser277.
免疫原
peptide corresponding to amino acids surrounding Thr275/Ser277 of human MKK7/SKK4.
应用
Anti-Phospho-MKK7/SKK4 (Thr275/Ser277), Cat. No. 36-010, is a highly specific rabbit polyclonal antibody that detect dual specificity mitogen-activated protein kinase kinase 7 (MKK7) and has been tested for use in Western Blotting.
质量
Evaluated by Western Blotting in lysate of A431 cells treates with Calyculin A and okadaic acid.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Phospho-MKK7/SKK4 (Thr275/Ser277) in lysate from 10 µg of A431 cells treated with Calyculin A and okadaic acid.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Phospho-MKK7/SKK4 (Thr275/Ser277) in lysate from 10 µg of A431 cells treated with Calyculin A and okadaic acid.
目标描述
~47kDa
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
K Yoshida et al.
Molecular and cellular biology, 20(15), 5370-5380 (2000-07-13)
The cellular response to DNA damage includes activation of the nuclear Lyn protein tyrosine kinase. Using cells deficient in Lyn expression, the present studies demonstrate that Lyn is required in part for induction of the stress-activated protein kinase (SAPK) in
I N Foltz et al.
The Journal of biological chemistry, 273(15), 9344-9351 (1998-05-16)
We report the cloning of a novel human activator of c-Jun N-terminal kinase (JNK), mitogen-activated protein kinase kinase 7 (MKK7). The mRNA for MKK7 is widely expressed in humans and mice and encodes a 47-kDa protein (419 amino acids), as
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