产品名称
内切蛋白酶 Lys-C,测序级,产酶溶杆菌,
grade
Proteomics Grade
assay
≥90% (SDS-PAGE)
form
lyophilized
specific activity
≥200 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
shipped in
ambient
storage temp.
2-8°C
Quality Level
Disclaimer
毒性:有害(C)
General description
来自产酶溶杆菌的天然胞内蛋白酶Lys-C。一种丝氨酸蛋白酶,能特异性水解肽类和蛋白质类中赖氨酸羧基侧的酰胺和肽酯键。用于蛋白测序或序列确认、蛋白结构域分析和融合蛋白的裂解。受抑肽酶、DFP、亮抑蛋白酶肽和TLCK抑制。建议的工作浓度:1:20至1:100(按重量计,蛋白酶:蛋白质)用于序列分析。
Other Notes
一个单位定义为在25°C,pH 7.7下,每分钟水解1.0 µmol Tos-Gly-Pro-Lys-pNA的酶量。
Physical form
从50 mM HEPES,10 mM EDTA,5 mg/ml棉子糖(pH 8.0)中冻干。
Preparation Note
在50 µl蒸馏H₂O中复溶。
复溶后,等分并冷冻长期保存(-20°C),或冷藏(4°C)短期保存。储备溶液在4°C下可稳定保存2天,在-20°C下可稳定保存1个月。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
S Zhang et al.
The Journal of biological chemistry, 271(52), 33575-33579 (1996-12-27)
While multiple G protein beta and gamma subunit isoforms have been identified, the implications of this potential diversity of betagamma heterodimers for signaling through betagamma-regulated effector pathways remains unclear. Furthermore the molecular mechanism(s) by which the betagamma complex modulates diverse
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