质量水平
制造商/商品名称
Upstate®
技术
activity assay: suitable (phosphatase)
NCBI登记号
UniProt登记号
检测方法
colorimetric
运输
wet ice
基因信息
human ... PTPN1(5770)
应用
PTP Assay Kit 1 can be used to detect PTP-1B activity by dephosphorylation of the phosphopeptide (RRLIEDAEpYAARG) using malachite green detection, or by hydrolysis of pNPP.
Used to detect/quantify: PTP
包装
Kit capacity: 100 assays
组分
Tyrosine Phosphopeptide (RRLIEDAEpYAARG] (Cat.# 12-217)
Malachite Green Solution A (Cat.# 20-105)
Malachite Green Additive (Cat.# 20-104)
Phosphate Standard (Cat.# 20-103)
pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
pNPP Tyr Assay Buffer (Cat.# 20-180)
96-well microtiter plate
Malachite Green Solution A (Cat.# 20-105)
Malachite Green Additive (Cat.# 20-104)
Phosphate Standard (Cat.# 20-103)
pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
pNPP Tyr Assay Buffer (Cat.# 20-180)
96-well microtiter plate
质量
routinely evaluated by malachite green and pNPP hydrolysis assays to detect PTP-1B (Catalog # 14-109 or Catalog # 14-358) activity by dephosphorylation of the phosphopeptide
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
警示用语：
Warning
危险声明
危险分类
Aquatic Chronic 3 - Met. Corr. 1
WGK
WGK 3
Involvement of Src-homology-2-domain-containing protein-tyrosine phosphatase 2 in T cell activation.
P Tailor et al.
European journal of biochemistry, 237(3), 736-742 (1996-05-01)
Activation of resting T lymphocytes by ligands to the complex of T cell antigen receptor (TCR) and CD3 is initiated by a series of critical tyrosine phosphorylation and dephosphorylation events. Protein-tyrosine kinases of the Syk, Src and Csk families and
Wenbin Xiao et al.
Blood, 116(26), 6003-6013 (2010-09-23)
Hyperactivation of the transcription factor Stat5 leads to various leukemias. Stat5 activity is regulated by the protein phosphatase SHP-1 in a phospholipase C (PLC)-β3-dependent manner. Thus, PLC-β3-deficient mice develop myeloproliferative neoplasm, like Lyn (Src family kinase)- deficient mice. Here we
A Hochstenbach-Waelen et al.
Physiology & behavior, 101(2), 237-244 (2010-05-12)
In the short-term, gelatin showed stronger hunger suppression and less energy intake compared with other proteins. This study investigated if a supra-sustained gelatin-milk protein (GMP) diet improves weight maintenance (WM) compared with a sustained milk protein (SMP) diet and supra-sustained
P A Ram et al.
The Journal of biological chemistry, 272(28), 17694-17702 (1997-07-11)
Growth hormone (GH) rapidly stimulates tyrosine phosphorylation followed by serine/threonine phosphorylation of multiple cytoplasmic STAT transcription factors, including one, STAT5b, that is uniquely responsive to the temporal pattern of plasma GH stimulation in rat liver and is proposed to play
Tamer Ahmed et al.
American journal of physiology. Gastrointestinal and liver physiology, 291(1), G35-G44 (2006-04-01)
Growth hormone (GH) stimulates STAT5 phosphorylation by JAK2, which activates IGF-I and serine protease inhibitor 2.1 (Spi 2.1) transcription, whereas STAT5 dephosphorylation by protein tyrosine phosphatases (PTPs) terminates this signal. We hypothesized that the inhibitory effects of TNF on GH
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