Tie2 (Y897S) Protein, active

N-terminal 6His-tagged recombinant human Tie2 residues 771-end.

The Y897S substitution is a gain-of-function mutation associated with hereditary venous malformations (Calvert et al., (1999), Hum. Mol. Genet. 8, 1279-1289).

Also available in 250μg size-call for pricing and availability and reference catalog number 14-764M when ordering the 250μg size.

Also available in 10 μg size (cat#14-764) and in bulk (cat# 14-764-K).

routinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1)

42kDa

50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap. Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.