ligand
(Carboxyethyl )
质量水平
物料
polypropylene housing
产品线
Fractogel®
形式
prepacked column
参数
≤8 bar pressure
~300 cm/hr max. flow rate
column I.D. × L
8 mm × 20 mm
体积
1 mL
基质活性基团
Methacrylate
平均粒径
40-90 μm
容量
~100 mL, resin binding capacity
应用
mAb
recombinant protein
vaccine development
分离技术
cation exchange
储存温度
15-25°C
一般描述
8 x 20mm
特点和优势
MiniChrom Column Fractogel® COO (M), 1 ml enables:
Due to the titration behavior, the ion exchange capacity can be used from pH 6 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD COO- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).
The strength of the binding depends on the following:
- Good resolution between monomers and aggregates
- Efficient aggregate and HCP removal combined with high yield
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior, the ion exchange capacity can be used from pH 6 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD COO- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the degree of the ionization of the functional groups of the exchanger
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
分析说明
Appearance: passes test
法律信息
FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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